Academic Journal
Efficient prime editing in two-cell mouse embryos using PEmbryo
| العنوان: | Efficient prime editing in two-cell mouse embryos using PEmbryo |
|---|---|
| المؤلفون: | Kim-Yip, Rebecca P., McNulty, Ryan, Joyce, Bradley, Mollica, Antonio, Chen, Peter J., Ravisankar, Purnima, Law, Benjamin K., Liu, David R., Toettcher, Jared E., Ivakine, Evgueni A., Posfai, Eszter, Adamson, Britt |
| المصدر: | Nature Biotechnology ; ISSN 1087-0156 1546-1696 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2024 |
| الوصف: | Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequency across 13 substitution edits at 8 sites), enabling same-generation phenotyping of founders. Whole-genome sequencing reveals that mismatch repair inhibition increases off-target indels at low-complexity regions in the genome without any obvious phenotype in mice. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41587-023-02106-x |
| الاتاحة: | http://dx.doi.org/10.1038/s41587-023-02106-x https://www.nature.com/articles/s41587-023-02106-x.pdf https://www.nature.com/articles/s41587-023-02106-x |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.F0CED112 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41587-023-02106-x |
|---|