Academic Journal
Microchip capillary electrophoresis-mass spectrometry for high-throughput simultaneous analysis of B-complex vitamins.
| العنوان: | Microchip capillary electrophoresis-mass spectrometry for high-throughput simultaneous analysis of B-complex vitamins. |
|---|---|
| المؤلفون: | Yang, Liu, Wang, Shuang, Li, Xiaolong, Wang, Weimin, Xu, Fuxing, Ding, Chuan-Fan |
| المصدر: | J Chromatogr A ; ISSN:1873-3778 ; Volume:1740 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2025 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | B-complex vitamins, Microchip capillary electrophoresis-mass spectrometry, Simultaneous analysis, ZipChip |
| الوصف: | B-complex vitamins are essential micronutrients that maintaining health, and provide (individually/simultaneously) many important biological actions in organism. Therefore, sensitive, reliable analytical method to determine B-complex vitamins simultaneously in actual samples is significant. Conventional analytical methods for vitamins analysis are usually labor-intensive, time-consuming and mostly do not allow the simultaneous determination. Few papers have been published on capillary electrophoresis-mass spectrometry (CE-MS) analysis of individual vitamin B or B-complex vitamins. Microchip capillary electrophoresis (ME) offers less consumption of background electrolyte and reagents than CE, and fast separation as having several channels for high-throughput. ME based on ZipChip integrates sample injection, electrophoretic separation, and ESI functions into one platform, herein, for the first time, in this paper, the ME-MS based on ZipChip platform was investigated and demonstrated preliminarily a significant improvement over existing techniques. Because: the ME-MS method has a linear response with excellent precision for replicate injections and LODs lower than 4 ppb; The method is simple and fast (<3 min per sample), using a small amount of sample (∼5 nL) with low reagent consumption, making it suitable for high-throughput applications; The inter/intraday reproducibility (RSD %) is less than 3 %, which is important for clinic application. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doi.org/10.1016/j.chroma.2024.465589; https://pubmed.ncbi.nlm.nih.gov/39662335 |
| DOI: | 10.1016/j.chroma.2024.465589 |
| الاتاحة: | https://doi.org/10.1016/j.chroma.2024.465589 https://pubmed.ncbi.nlm.nih.gov/39662335 |
| Rights: | Copyright © 2024 Elsevier B.V. All rights reserved. |
| رقم الانضمام: | edsbas.F017E817 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.chroma.2024.465589 |
|---|