Academic Journal
CD8 + T cell immunity Is compromised by Anti-CD20 treatment and rescued by Interleukin-17A
| العنوان: | CD8 + T cell immunity Is compromised by Anti-CD20 treatment and rescued by Interleukin-17A |
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| المؤلفون: | Fiocca Vernengo, Facundo, Beccaria, Cristian Gabriel, Araujo Furlan, Cintia Liliana, Tosello Boari, Jimena, Almada, Laura, Gorosito Serran, Melisa, Gazzoni, Yamila Natali, Montes, Carolina Lucia, Acosta Rodriguez, Eva Virginia, Gruppi, Adriana |
| بيانات النشر: | ASM |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | TRYPANOSOMA CRUZI, CD8 T CELL, ANTI-CD20, B CELL, https://purl.org/becyt/ford/3.3, https://purl.org/becyt/ford/3 |
| الوصف: | Treatment with anti-CD20, used in many diseases in which B cells play a pathogenic role, has been associated with susceptibility to intracellular infections. Here, we studied the effect of anti-CD20 injection on CD8+ T cell immunity using an experimental model of Trypanosoma cruzi infection, in which CD8+ T cells play a pivotal role. C57BL/6 mice were treated with anti-CD20 for B cell depletion prior to T. cruzi infection. Infected anti-CD20-treated mice exhibited a CD8+ T cell response with a conserved expansion phase followed by an early contraction, resulting in a strong reduction in total and parasite-specific CD8+ T cell numbers at 20 days postinfection. Anti-CD20 injection increased the frequency of apoptotic CD8+ T cells, decreased the number of effector and memory CD8+ T cells, and reduced the frequency of proliferating and cytokine-producing CD8+ T cells. Accordingly, infected anti-CD20-treated mice presented lower cytotoxicity of T. cruzi peptide-pulsed target cells in vivo. All of these alterations in CD8+ T cell immunity were associated with increased tissue parasitism. Anti-CD20 injection also dampened the CD8+ T cell response, when this had already been generated, indicating that B cells were involved in the maintenance rather than the induction of CD8+ T cell immunity. Anti-CD20 injection also resulted in a marked reduction in the frequency of interleukin-6 (IL-6)- and IL-17A-producing cells, and recombinant IL-17A (rIL-17A) injection partially restored the CD8+ T cell response in infected anti-CD20-treated mice. Thus, anti-CD20 reduced CD8+ T cell immunity, and IL-17A is a candidate for rescuing deficient responses either directly or indirectly. ; Fil: Fiocca Vernengo, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina ; Fil: Beccaria, Cristian Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2161-2129 2150-7511 |
| Relation: | http://hdl.handle.net/11336/159229; Fiocca Vernengo, Facundo; Beccaria, Cristian Gabriel; Araujo Furlan, Cintia Liliana; Tosello Boari, Jimena; Almada, Laura; et al.; CD8 + T cell immunity Is compromised by Anti-CD20 treatment and rescued by Interleukin-17A; ASM; mBio; 11; 3; 5-2020; 1-17; CONICET Digital; CONICET |
| الاتاحة: | http://hdl.handle.net/11336/159229 |
| Rights: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| رقم الانضمام: | edsbas.F0124227 |
| قاعدة البيانات: | BASE |
| تدمد: | 21612129 21507511 |
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