Academic Journal
TAPBPR mediates peptide dissociation from MHC class I using a leucine lever
| العنوان: | TAPBPR mediates peptide dissociation from MHC class I using a leucine lever |
|---|---|
| المؤلفون: | Ilca, F Tudor, Neerincx, Andreas, Hermann, Clemens, Marcu, Ana, Stevanović, Stefan, Deane, Janet E, Boyle, Louise H |
| المساهمون: | Wellcome Trust, South African Medical Research Council, Royal Society, Bosch-Forschungsstiftung |
| المصدر: | eLife ; volume 7 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2018 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | Tapasin and TAPBPR are known to perform peptide editing on major histocompatibility complex class I (MHC I) molecules; however, the precise molecular mechanism(s) involved in this process remain largely enigmatic. Here, using immunopeptidomics in combination with novel cell-based assays that assess TAPBPR-mediated peptide exchange, we reveal a critical role for the K22-D35 loop of TAPBPR in mediating peptide exchange on MHC I. We identify a specific leucine within this loop that enables TAPBPR to facilitate peptide dissociation from MHC I. Moreover, we delineate the molecular features of the MHC I F pocket required for TAPBPR to promote peptide dissociation in a loop-dependent manner. These data reveal that chaperone-mediated peptide editing on MHC I can occur by different mechanisms dependent on the C-terminal residue that the MHC I accommodates in its F pocket and provide novel insights that may inform the therapeutic potential of TAPBPR manipulation to increase tumour immunogenicity. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.40126 |
| الاتاحة: | http://dx.doi.org/10.7554/elife.40126 https://cdn.elifesciences.org/articles/40126/elife-40126-v2.pdf https://cdn.elifesciences.org/articles/40126/elife-40126-v2.xml https://elifesciences.org/articles/40126 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.EFB902EE |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.40126 |
|---|