Academic Journal
Flow cytometry for fast screening and automated risk assessment in systemic light-chain amyloidosis
| العنوان: | Flow cytometry for fast screening and automated risk assessment in systemic light-chain amyloidosis |
|---|---|
| المؤلفون: | Puig, Noemi, Paiva, Bruno, Lasa, Marta, Burgos, Leire, Pérez, José J., Merino, Juana, Moreno, Cristina, Vidriales, Maria Belén, Gómez Toboso, Dolores, Cedena, Maria-Teresa, Ocio, Enrique M., Lécumberri, Ramon, García de Coca, Alfonso, Labrador, Jorge, Gonzalez, Maria-Esther, Palomera, Luis, Gironella, Mercedes, Cabañas, Valentin, Casanova, María, Oriol, Albert, Krsnik, Isabel, Pérez-Montaña, Albert, Rubia, Javier de la, Puerta, José Enrique de la, Arriba, Felipe de, Prosper, Felipe, Martínez-López, Joaquín, Lécrevisse, Quentin, Verde, Javier, Mateos, Maria Victoria, Lahuerta, Juan José, Orfao, Alberto, San Miguel, Jesús F. |
| المساهمون: | Instituto de Salud Carlos III, Asociación Española Contra el Cáncer, International Myeloma Foundation, European Research Council |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Haematological cancer, Myeloma, Translational research |
| الوصف: | Early diagnosis and risk stratification are key to improve outcomes in light-chain (AL) amyloidosis. Here we used multidimensional-flow-cytometry (MFC) to characterize bone marrow (BM) plasma cells (PCs) from a series of 166 patients including newly-diagnosed AL amyloidosis (N = 94), MGUS (N = 20) and multiple myeloma (MM, N = 52) vs. healthy adults (N = 30). MFC detected clonality in virtually all AL amyloidosis (99%) patients. Furthermore, we developed an automated risk-stratification system based on BMPCs features, with independent prognostic impact on progression-free and overall survival of AL amyloidosis patients (hazard ratio: ≥ 2.9;P ≤.03). Simultaneous assessment of the clonal PCs immunophenotypic protein expression profile and the BM cellular composition, mapped AL amyloidosis in the crossroad between MGUS and MM; however, lack of homogenously-positive CD56 expression, reduction of B-cell precursors and a predominantly-clonal PC compartment in the absence of an MM-like tumor PC expansion, emerged as hallmarks of AL amyloidosis (ROC-AUC = 0.74;P <.001), and might potentially be used as biomarkers for the identification of MGUS and MM patients, who are candidates for monitoring pre-symptomatic organ damage related to AL amyloidosis. Altogether, this study addressed the need for consensus on how to use flow cytometry in AL amyloidosis, and proposes a standardized MFC-based automated risk classification ready for implementation in clinical practice. ; This study was supported by the Centro de Investigación Biomédica en Red – Área de Oncología—del Instituto de Salud Carlos III (CIBERONC; CB16/12/00369, CB16/12/00400 and CB16/12/00489), Instituto de Salud Carlos III/Subdirección General de Investigación Sanitaria (FIS No. PI13/02196), Asociación Española Contra el Cáncer (GCB120981SAN and Accelerator Award), the Black Swan Research Initiative of the International Myeloma Foundation, and the European Research Council (ERC) 2015 Starting Grant (MYELOMANEXT). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0887-6924 1476-5551 |
| Relation: | http://dx.doi.org/10.1038/s41375-018-0308-5; Sí; Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 33: 1256-1267 (2019); http://hdl.handle.net/10261/203219; http://dx.doi.org/10.13039/501100004587; http://dx.doi.org/10.13039/100003887; http://dx.doi.org/10.13039/501100000781 |
| DOI: | 10.1038/s41375-018-0308-5 |
| DOI: | 10.13039/501100004587 |
| DOI: | 10.13039/100003887 |
| DOI: | 10.13039/501100000781 |
| الاتاحة: | http://hdl.handle.net/10261/203219 https://doi.org/10.1038/s41375-018-0308-5 https://doi.org/10.13039/501100004587 https://doi.org/10.13039/100003887 https://doi.org/10.13039/501100000781 |
| Rights: | none |
| رقم الانضمام: | edsbas.EF46EFB0 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 08876924 14765551 |
|---|---|
| DOI: | 10.1038/s41375-018-0308-5 |