التفاصيل البيبلوغرافية
| العنوان: |
Early IL-10 signaling is critical for humoral immunity. |
| المؤلفون: |
Fionna A. Surette (10074266), Jenna J. Guthmiller (3211731), Lei Li (29537), Alexandria J. Sturtz (10074269), Rahul Vijay (10074272), Rosemary L. Pope (3211728), Brandon L. McClellan (10074275), Angela D. Pack (5954858), Ryan A. Zander (3211734), Peng Shao (248983), Linda Yu-Ling Lan (10074278), Daniel Fernandez-Ruiz (567632), William R. Heath (10074281), Patrick C. Wilson (8708271), Noah S. Butler (10074284) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Ecology, Immunology, Developmental Biology, Cancer, Infectious Diseases, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, CD 4 T cells, Foxp 3-negative effector CD 4 T cells, Plasmodium humoral immunity Immunity, MHC class II, B cell-intrinsic manner, B cell survival, GC B cell responses, blood-stage Plasmodium infection, Extrafollicular CD 4 T cell-derived., malaria, cytokine IL -10, B cell expression, IL -10, B cell follicles, cell-cell adhesion proteins, CD 4 T-cells, support humoral immunity, TFH |
| الوصف: |
( A-C ) P . yoelii infected WT mice were administered α-IL-10R from either days 1–7 p.i. (n = 6), days 7–13 p.i. (n = 7) or rIgG (n = 7). Parasitemia ( A ), parasite lysate-specific end point titers on day 18 p.i. ( B ) and survival ( C ) were evaluated. Data in A-C (means ± S.E.M.) represent n = 3–4 mice/group pooled from 2 independent experiments. * P ≤ 0.05 and **** P ≤ 0.0001 comparing rIgG to α-IL-10R (d1-7). Differences between rIgG and α-IL-10R (d7-13) were not significant in any assay. ( D-G ) Representative plots and summary data showing numbers of GC-TFH cells ( D,E ) and GC B cells ( F,G ) that develop when IL-10R signaling is blocked on the indicated days p.i. ( H ) Groups of WT C57BL/6 mice (n = 3/time point) were infected with P . yoelii and on day 6 and 10 p.i. spleens were sectioned and stained with α-B220-AF488, clone GL-7-biotin, α-CD4-AF647. The sections were then counter-stained with streptavidin-BV421. Images were processed using IMARIS software. Data in A were analyzed by Log-rank (Mantel-Cox) test. Data in B were analyzed using unpaired, non-parametric Kruskal-Wallis tests correcting for multiple comparisons using the Dunn’s test. Data in E and G were analyzed using one-way ANOVA and Dunnett’s multiple comparison tests, with each group compared to rIgG. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P < 0.0001. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Early_IL-10_signaling_is_critical_for_humoral_immunity_/13695503 |
| DOI: |
10.1371/journal.ppat.1009288.g002 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1009288.g002 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.EEAD7AD4 |
| قاعدة البيانات: |
BASE |