Academic Journal
HCG18, LEF1AS1 and lncCEACAM21 as biomarkers of disease severity in the peripheral blood mononuclear cells of COVID-19 patients
| العنوان: | HCG18, LEF1AS1 and lncCEACAM21 as biomarkers of disease severity in the peripheral blood mononuclear cells of COVID-19 patients |
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| المؤلفون: | Greco, Simona, Made’, Alisia, Mutoli, Martina, Zhang, Lu, Piella, Santiago Nicolas, Vausort, Mélanie, Lumley, Andrew I., Beltrami, Antonio Paolo, Srivastava, Prashant Kumar, Milani, Valentina, Boveri, Sara, Ranucci, Marco, Renna, Laura Valentina, Firat, Hüseyin, Bruno, Antonino, Spinetti, Gaia, Emanueli, Costanza, Devaux, Yvan, Martelli, Fabio |
| المساهمون: | Ministero della Salute, Ministero della cultura, Fondazione Telethon, AFM-Téléthon, European Commission, Horizon 2020 Framework Programme, Fonds National de la Recherche Luxembourg, Ministère de l'Enseignement Supérieur et de la Recherche, Associazione Italiana per la Ricerca sul Cancro, Fondazione Cariplo, British Heart Foundation |
| المصدر: | Journal of Translational Medicine ; volume 21, issue 1 ; ISSN 1479-5876 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| الوصف: | Background Even after 3 years from SARS-CoV-2 identification, COVID-19 is still a persistent and dangerous global infectious disease. Significant improvements in our understanding of the disease pathophysiology have now been achieved. Nonetheless, reliable and accurate biomarkers for the early stratification of COVID-19 severity are still lacking. Long noncoding RNAs (LncRNAs) are ncRNAs longer than 200 nucleotides, regulating the transcription and translation of protein‐coding genes and they can be found in the peripheral blood, thus holding a promising biomarker potential. Specifically, peripheral blood mononuclear cells (PBMCs) have emerged as a source of indirect biomarkers mirroring the conditions of tissues: they include monocytes, B and T lymphocytes, and natural killer T cells (NKT), being highly informative for immune-related events. Methods We profiled by RNA-Sequencing a panel of 2906 lncRNAs to investigate their modulation in PBMCs of a pilot group of COVID-19 patients, followed by qPCR validation in 111 hospitalized COVID-19 patients. Results The levels of four lncRNAs were found to be decreased in association with COVID-19 mortality and disease severity: HLA Complex Group 18-242 and -244 (HCG18-242 and HCG18-244), Lymphoid Enhancer Binding Factor 1-antisense 1 (LEF1-AS1) and lncCEACAM21 (i.e. ENST00000601116.5, a lncRNA in the CEACAM21 locus). Interestingly, these deregulations were confirmed in an independent patient group of hospitalized patients and by the re-analysis of publicly available single-cell transcriptome datasets. The identified lncRNAs were expressed in all of the PBMC cell types and inversely correlated with the neutrophil/lymphocyte ratio (NLR), an inflammatory marker. In vitro, the expression of LEF1-AS1 and lncCEACAM21 was decreased upon THP-1 monocytes exposure to a relevant stimulus, hypoxia. Conclusion The identified COVID-19-lncRNAs are proposed as potential innovative biomarkers of COVID-19 severity and mortality. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s12967-023-04497-6 |
| DOI: | 10.1186/s12967-023-04497-6.pdf |
| DOI: | 10.1186/s12967-023-04497-6/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s12967-023-04497-6 https://link.springer.com/content/pdf/10.1186/s12967-023-04497-6.pdf https://link.springer.com/article/10.1186/s12967-023-04497-6/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.EDF47D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12967-023-04497-6 |
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