Academic Journal
Detection of HIV-1 Transmission Clusters from Dried Blood Spots within a Universal Test-and-Treat Trial in East Africa.
| العنوان: | Detection of HIV-1 Transmission Clusters from Dried Blood Spots within a Universal Test-and-Treat Trial in East Africa. |
|---|---|
| المؤلفون: | Pujol-Hodge, Emma, Salazar-Gonzalez, Jesus F, Ssemwanga, Deogratius, Charlebois, Edwin D, Ayieko, James, Grant, Heather E, Liegler, Teri, Atkins, Katherine E, Kaleebu, Pontiano, Kamya, Moses R, Petersen, Maya, Havlir, Diane V, Leigh Brown, Andrew J |
| بيانات النشر: | MDPI |
| سنة النشر: | 2022 |
| المجموعة: | London School of Hygiene & Tropical Medicine: LSHTM Research Online |
| الوصف: | The Sustainable East Africa Research in Community Health (SEARCH) trial was a universal test-and-treat (UTT) trial in rural Uganda and Kenya, aiming to lower regional HIV-1 incidence. Here, we quantify breakthrough HIV-1 transmissions occurring during the trial from population-based, dried blood spot samples. Between 2013 and 2017, we obtained 549 gag and 488 pol HIV-1 consensus sequences from 745 participants: 469 participants infected prior to trial commencement and 276 SEARCH-incident infections. Putative transmission clusters, with a 1.5% pairwise genetic distance threshold, were inferred from maximum likelihood phylogenies; clusters arising after the start of SEARCH were identified with Bayesian time-calibrated phylogenies. Our phylodynamic approach identified nine clusters arising after the SEARCH start date: eight pairs and one triplet, representing mostly opposite-gender linked (6/9), within-community transmissions (7/9). Two clusters contained individuals with non-nucleoside reverse transcriptase inhibitor (NNRTI) resistance, both linked to intervention communities. The identification of SEARCH-incident, within-community transmissions reveals the role of unsuppressed individuals in sustaining the epidemic in both arms of a UTT trial setting. The presence of transmitted NNRTI resistance, implying treatment failure to the efavirenz-based antiretroviral therapy (ART) used during SEARCH, highlights the need to improve delivery and adherence to up-to-date ART recommendations, to halt HIV-1 transmission. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://researchonline.lshtm.ac.uk/id/eprint/4667204/1/Hodge_etal_2022_Detection-of-hiv-1-transmission.pdf; Pujol-Hodge, Emma; Salazar-Gonzalez, Jesus F; Ssemwanga, Deogratius ; Charlebois, Edwin D; Ayieko, James; Grant, Heather E; Liegler, Teri; Atkins, Katherine E ; Kaleebu, Pontiano ; Kamya, Moses R; +3 more. Petersen, Maya; Havlir, Diane V; Leigh Brown, Andrew J; (2022) Detection of HIV-1 Transmission Clusters from Dried Blood Spots within a Universal Test-and-Treat Trial in East Africa. Viruses, 14 (8). p. 1673. ISSN 1999-4915 DOI: https://doi.org/10.3390/v14081673 |
| الاتاحة: | https://researchonline.lshtm.ac.uk/id/eprint/4667204/ https://researchonline.lshtm.ac.uk/id/eprint/4667204/1/Hodge_etal_2022_Detection-of-hiv-1-transmission.pdf |
| Rights: | cc_by_4 |
| رقم الانضمام: | edsbas.ED93B2CA |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |