Academic Journal
Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model
| العنوان: | Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model |
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| المؤلفون: | Rutherford, Holly, Candeias, Diogo, Duncan, Christopher J A, Renshaw, Stephen A, Hamilton, Noemie Magali Renee |
| سنة النشر: | 2024 |
| المجموعة: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
| الوصف: | RNASET2-deficient leukodystrophy is a rare infantile white matter disorder mimicking a viral infection and resulting in severe psychomotor impairments. Despite its severity, there is little understanding of cellular mechanisms of pathogenesis and no treatments. Recent research using the rnaset2 mutant zebrafish model has suggested that microglia may be the drivers of the neuropathology, due to their failure to digest apoptotic debris during neurodevelopment. Therefore, we developed a strategy for microglial replacement through transplantation of adult whole kidney marrow-derived macrophages into embryonic hosts. Using live imaging, we revealed that transplant-derived macrophages can engraft within host brains and express microglia-specific markers, suggesting adoption of a microglial phenotype. Tissue clearing strategies revealed the persistence of transplanted cells in host brains beyond embryonic stages. We demonstrated that transplanted cells clear apoptotic cells within the brain, as well as rescuing overactivation of the antiviral response otherwise seen in mutant larvae. RNA sequencing at the point of peak transplant-derived cell engraftment confirms that transplantation can reduce the brain-wide immune response, and particularly the antiviral response, in rnaset2-deficient brains. Crucially, this reduction in neuroinflammation resulted in behavioural rescue - restoring rnaset2 mutant motor activity to wild type levels in embryonic and juvenile stages. Together, these findings demonstrate the role of microglia as the cellular drivers of neuropathology in rnaset2 mutants, and that macrophage transplantation is a viable strategy for microglial replacement in the zebrafish. Therefore, microglia-targeted interventions may have therapeutic benefits in RNASET2-deficient leukodystrophy. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://eprints.whiterose.ac.uk/212498/8/rutherford-et-al-2024-macrophage-transplantation-rescues-rnaset2-deficient-leukodystrophy-by-replacing-deficient.pdf; Rutherford, Holly, Candeias, Diogo, Duncan, Christopher J A et al. (2 more authors) (2024) Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model. PNAS. ISSN 1091-6490 |
| DOI: | 10.1073/pnas.2321496121 |
| الاتاحة: | https://eprints.whiterose.ac.uk/212498/ https://doi.org/10.1073/pnas.2321496121 |
| Rights: | cc_by |
| رقم الانضمام: | edsbas.ED8F9ADE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1073/pnas.2321496121 |
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