Academic Journal
Pharyngeal Communities and Antimicrobial Resistance in Pangolins in Gabon
| العنوان: | Pharyngeal Communities and Antimicrobial Resistance in Pangolins in Gabon |
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| المؤلفون: | Johanna P. Wiethoff, Sarah Sandmann, Tom Theiler, Chimene Nze Nkogue, Etienne-François Akomo-Okoue, Julian Varghese, Andrea Kreidenweiss, Alexander Mellmann, Bertrand Lell, Ayôla A. Adegnika, Jana Held, Frieder Schaumburg |
| المصدر: | Microbiology Spectrum, Vol 11, Iss 4 (2023) |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Africa, ESBL, pangolin, antimicrobial resistance, microbiome, Microbiology, QR1-502 |
| الوصف: | Wildlife can be a reservoir and source of zoonotic pathogens for humans. For instance, pangolins were considered one of the potential animal reservoirs of SARS-CoV-2. The aim of this study was to assess the prevalence of antimicrobial-resistant species (e.g., extended-spectrum β-lactamase [ESBL]-producing Enterobacterales) and Staphylococcus aureus-related complex and to describe the bacterial community in wild Gabonese pangolins. The pharyngeal colonization of pangolins sold in Gabon (n = 89, 2021 to 2022) was analyzed using culture media selective for ESBL-producing Enterobacterales, S. aureus-related complex, Gram-positive bacteria and nonfermenters. Phylogenetic analyses of ESBL-producing Enterobacterales was done using core-genome multilocus sequence typing (cgMLST) and compared with publicly available genomes. Patterns of cooccurring species were detected by network analysis. Of the 439 bacterial isolates, the majority of species belonged to the genus Pseudomonas (n = 170), followed by Stenotrophomonas (n = 113) and Achromobacter (n = 37). Three Klebsiella pneumoniae isolates and one Escherichia coli isolate were ESBL-producers, which clustered with human isolates from Nigeria (MLST sequence type 1788 [ST1788]) and Gabon (ST38), respectively. Network analysis revealed a frequent cooccurrence of Stenotrophomonas maltophilia with Pseudomonas putida and Pseudomonas aeruginosa. In conclusion, pangolins can be colonized with human-related ESBL-producing K. pneumoniae and E. coli. Unlike in other African wildlife, S. aureus-related complex was not detected in pangolins. IMPORTANCE There is an ongoing debate if pangolins are a relevant reservoir for viruses such as SARS-CoV-2. Here, we wanted to know if African pangolins are colonized with bacteria that are relevant for human health. A wildlife reservoir of antimicrobial resistance would be of medical relevance in regions were consumption of so-called bushmeat is common. In 89 pangolins, we found three ESBL-producing Klebsiella pneumoniae strains and one ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2165-0497 |
| Relation: | https://doaj.org/toc/2165-0497; https://doaj.org/article/064d9fb85d964627b4f08006b43dec9b |
| DOI: | 10.1128/spectrum.00664-23 |
| الاتاحة: | https://doi.org/10.1128/spectrum.00664-23 https://doaj.org/article/064d9fb85d964627b4f08006b43dec9b |
| رقم الانضمام: | edsbas.ED16C87 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21650497 |
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| DOI: | 10.1128/spectrum.00664-23 |