Academic Journal
The readthrough variant of acetylcholinesterase remains very minor after heat shock, organophosphate inhibition and stress, in cell culture and in vivo
| العنوان: | The readthrough variant of acetylcholinesterase remains very minor after heat shock, organophosphate inhibition and stress, in cell culture and in vivo |
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| المؤلفون: | Perrier, Noël A., Salani, Monica, Falasca, Cinzia, Bon, Suzanne, Augusti‐Tocco, Gabriella, Massoulié, Jean |
| المصدر: | Journal of Neurochemistry ; volume 94, issue 3, page 629-638 ; ISSN 0022-3042 1471-4159 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2005 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Acetylcholinesterase (AChE) exists in various molecular forms, depending on alternative splicing of its transcripts and association with structural proteins. Tetramers of the ‘tailed’ variant (AChE T ), which are anchored in the cell membrane of neurons by the PRiMA ( P roline Ri ch M embrane A nchor) protein, constitute the main form of AChE in the mammalian brain. In the mouse brain, stress and anticholinesterase inhibitors have been reported to induce expression of the unspliced ‘readthrough’ variant (AChE R ) mRNA which produces a monomeric form. To generalize this observation, we attempted to quantify AChE R and AChE T after organophosphate intoxication in the mouse brain and compared the observed effects with those of stress induced by swimming or immobilization; we also analyzed the effects of heat shock and AChE inhibition on neuroblastoma cells. Active AChE molecular forms were characterized by sedimentation and non‐denaturing electrophoresis, and AChE transcripts were quantified by real‐time PCR. We observed a moderate increase of the AChE R transcript in some cases, both in the mouse brain and in neuroblastoma cultures, but we did not detect any increase of the corresponding active enzyme. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1471-4159.2005.03140.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1471-4159.2005.03140.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-4159.2005.03140.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.2005.03140.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.ECBE3DFA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1471-4159.2005.03140.x |
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