Academic Journal
A Comprehensive Evaluation of Nasal and Bronchial Cytokines and Chemokines Following Experimental Rhinovirus Infection in Allergic Asthma: Increased Interferons (IFN-γ and IFN-λ) and Type 2 Inflammation (IL-5 and IL-13)
| العنوان: | A Comprehensive Evaluation of Nasal and Bronchial Cytokines and Chemokines Following Experimental Rhinovirus Infection in Allergic Asthma: Increased Interferons (IFN-γ and IFN-λ) and Type 2 Inflammation (IL-5 and IL-13) |
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| المؤلفون: | Tunstall, T, Kon, OM, Bartlett, N, Hansel, TT, Johnston, SL, Mallia, P, Jackson, DJ, Walton, R, Edwards, M, Trujillo-Torralbo, MB, Del-Rosario, A, Shamji, B, Dhariwal, J, Kirk, P, Stumpf, M, Koopmann, JO, Telcian, A, Aniscenko, J, Gogsadze, L, Bakhsoliani, E, Stanciu, L, Hunt, TM, Hunt, TL, Hunt, DG, Westwick, J |
| المساهمون: | British Lung Foundation, Asthma UK, Commission of the European Communities, Medical Research Council (MRC), Imperial College Healthcare NHS Trust- BRC Funding |
| المصدر: | 138 ; 128 |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2017 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Absorption of mucosal lining fluid, Asthma, Interferons, Mucosal immunology, Rhinovirus, Type II inflammation |
| الوصف: | Background Rhinovirus infection is a major cause of asthma exacerbations. Objectives We studied nasal and bronchial mucosal inflammatory responses during experimental rhinovirus-induced asthma exacerbations. Methods We used nasosorption on days 0, 2–5 and 7 and bronchosorption at baseline and day 4 to sample mucosal lining fluid to investigate airway mucosal responses to rhinovirus infection in patients with allergic asthma (n = 28) and healthy non-atopic controls (n = 11), by using a synthetic absorptive matrix and measuring levels of 34 cytokines and chemokines using a sensitive multiplex assay. Results Following rhinovirus infection asthmatics developed more upper and lower respiratory symptoms and lower peak expiratory flows compared to controls (all P < 0.05). Asthmatics also developed higher nasal lining fluid levels of an anti-viral pathway (including IFN-γ, IFN-λ/IL-29, CXCL11/ITAC, CXCL10/IP10 and IL-15) and a type 2 inflammatory pathway (IL-4, IL-5, IL-13, CCL17/TARC, CCL11/eotaxin, CCL26/eotaxin-3) (area under curve day 0–7, all P < 0.05). Nasal IL-5 and IL-13 were higher in asthmatics at day 0 (P < 0.01) and levels increased by days 3 and 4 (P < 0.01). A hierarchical correlation matrix of 24 nasal lining fluid cytokine and chemokine levels over 7 days demonstrated expression of distinct interferon-related and type 2 pathways in asthmatics. In asthmatics IFN-γ, CXCL10/IP10, CXCL11/ITAC, IL-15 and IL-5 increased in bronchial lining fluid following viral infection (all P < 0.05). Conclusions Precision sampling of mucosal lining fluid identifies robust interferon and type 2 responses in the upper and lower airways of asthmatics during an asthma exacerbation. Nasosorption and bronchosorption have potential to define asthma endotypes in stable disease and at exacerbation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 2352-3964 |
| Relation: | EBioMedicine; http://hdl.handle.net/10044/1/45996; https://dx.doi.org/10.1016/j.ebiom.2017.03.033; P04/13; 02/027; 08/048; 233015; 260895; CH11SJ; G1000758; DHRBA_P26095 |
| DOI: | 10.1016/j.ebiom.2017.03.033 |
| الاتاحة: | http://hdl.handle.net/10044/1/45996 https://doi.org/10.1016/j.ebiom.2017.03.033 |
| Rights: | © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| رقم الانضمام: | edsbas.ECBE3577 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23523964 |
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| DOI: | 10.1016/j.ebiom.2017.03.033 |