Academic Journal
TrichoGate: An improved vector system for a large scale of functional analysis of Trichoderma genes
| العنوان: | TrichoGate: An improved vector system for a large scale of functional analysis of Trichoderma genes |
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| المؤلفون: | Nogueira Lopez, G, Padilla-Arizmendi, F, Inwood, S, Lyne, S, Steyaert, JM, Nieto-Jacobo, Maria, Stewart, A, Mendoza-Mendoza, A |
| بيانات النشر: | Frontiers Media |
| سنة النشر: | 2019 |
| المجموعة: | Lincoln University (New Zealand): Lincoln U Research Archive |
| مصطلحات موضوعية: | Golden Gate cloning technology, Trichoderma, Agrobacterium-mediated transformation, carboxin resistance, TrichoGate, functional genomics, synthetic biology, ANZSRC::0605 Microbiology, ANZSRC::060408 Genomics, ANZSRC::060113 Synthetic Biology, ANZSRC::3107 Microbiology, ANZSRC::3207 Medical microbiology |
| جغرافية الموضوع: | Switzerland |
| الوصف: | Species of the genus Trichoderma are ubiquitous in the environment and are widely used in agriculture, as biopesticides, and in the industry for the production of plant cell wall-degrading enzymes. Trichoderma represents an important genus of endophytes, and several Trichoderma species have become excellent models for the study of fungal biology and plant–microbe interactions; moreover, are exceptional biotechnological factories for the production of bioactive molecules useful in agriculture and medicine. Next generation sequencing technology coupled with systematic construction of recombinant DNA molecules provides powerful tools that contribute to the functional analysis of Trichoderma genetics, thus allowing for a better understanding of the underlying factors determining its biology. Here, we present the creation of diverse vectors containing (i) promoter-specific vectors for Trichoderma, (ii) gene deletions (using hygromycin phosphotransferase as selection marker), (iii) protein localization (mCherry and eGFP, which were codon-optimized for Trichoderma), (iv) gene complementation (neomycin phosphotransferase) and (v) overexpression of encoding gene proteins fused to fluorescent markers, by using the Golden Gate cloning technology. Furthermore, we present the design and implementation of a binary vector for Agrobacterium-mediated transformation in Trichoderma to increase the homologous recombination rate and the generation of a novel selection marker based on carboxin resistance. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 18 pages; Electronic-eCollection |
| اللغة: | English |
| تدمد: | 1664-302X |
| Relation: | The original publication is available from Frontiers Media - https://doi.org/10.3389/fmicb.2019.02794 - http://dx.doi.org/10.3389/fmicb.2019.02794; Frontiers in Microbiology; https://doi.org/10.3389/fmicb.2019.02794; https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000504423300001&DestLinkType=FullRecord&DestApp=WOS_CPL; JY4YT (isidoc); 31921006 (pubmed); https://hdl.handle.net/10182/11278 |
| DOI: | 10.3389/fmicb.2019.02794 |
| الاتاحة: | https://hdl.handle.net/10182/11278 https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=elements_prod&SrcAuth=WosAPI&KeyUT=WOS:000504423300001&DestLinkType=FullRecord&DestApp=WOS_CPL https://doi.org/10.3389/fmicb.2019.02794 |
| Rights: | © 2019 Nogueira-López, Padilla-Arizmendi, Inwood, Lyne, Steyaert, Nieto-Jacobo, Stewart and Mendoza-Mendoza ; Attribution ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.EC7DA6FC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1664302X |
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| DOI: | 10.3389/fmicb.2019.02794 |