التفاصيل البيبلوغرافية
| العنوان: |
Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods |
| المؤلفون: |
Pierella Karlusich, J., Pelletier, E., Lombard, F., Carsique, M., Dvorak, E., Colin, S., Picheral, M., Cornejo-Castillo, F., Acinas, S., Pepperkok, R., Karsenti, E., de Vargas, C., Wincker, P., Bowler, C., Foster, R. |
| المصدر: |
Nature Communications |
| سنة النشر: |
2021 |
| المجموعة: |
Max Planck Society: MPG.PuRe |
| الوصف: |
Nitrogen fixation has a critical role in marine primary production, yet our understanding of marine nitrogen-fixers (diazotrophs) is hindered by limited observations. Here, we report a quantitative image analysis pipeline combined with mapping of molecular markers for mining >2,000,000 images and >1300 metagenomes from surface, deep chlorophyll maximum and mesopelagic seawater samples across 6 size fractions (<0.2-2000 μm). We use this approach to characterise the diversity, abundance, biovolume and distribution of symbiotic, colony-forming and particle-associated diazotrophs at a global scale. We show that imaging and PCR-free molecular data are congruent. Sequence reads indicate diazotrophs are detected from the ultrasmall bacterioplankton (<0.2 μm) to mesoplankton (180-2000 μm) communities, while images predict numerous symbiotic and colony-forming diazotrophs (>20 µm). Using imaging and molecular data, we estimate that polyploidy can substantially affect gene abundances of symbiotic versus colony-forming diazotrophs. Our results support the canonical view that larger diazotrophs (>10 μm) dominate the tropical belts, while unicellular cyanobacterial and non-cyanobacterial diazotrophs are globally distributed in surface and mesopelagic layers. We describe co-occurring diazotrophic lineages of different lifestyles and identify high-density regions of diazotrophs in the global ocean. Overall, we provide an update of marine diazotroph biogeographical diversity and present a new bioimaging-bioinformatic workflow. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/34230473; http://hdl.handle.net/21.11116/0000-000A-3D01-1 |
| الاتاحة: |
http://hdl.handle.net/21.11116/0000-000A-3D01-1 |
| رقم الانضمام: |
edsbas.EC1DDAD7 |
| قاعدة البيانات: |
BASE |