التفاصيل البيبلوغرافية
| العنوان: |
Loop-Mediated Isothermal Amplifi cation PCR (LAMP-PCR) For Rapid Molecular Diagnosis of Group A Streptococci ; Grup A Streptokokların Hızlı Moleküler Tanısında Loop-Mediated Isothermal Amplifi cation PCR (LAMP-PCR) |
| المؤلفون: |
ALTINDİŞ, Mustafa, ELMAS, Bahri, KILIÇ, Ümit, ASLAN, Ferhat Gürkan, KÜÇÜKKARA, Gökhan, KÖROĞLU, Mehmet |
| المصدر: |
Volume: 1, Issue: 1 11-16 ; 2587-1641 ; Journal of Biotechnology and Strategic Health Research |
| بيانات النشر: |
Deneysel, Biyoteknolojik, Klinik ve Stratejik Sağlık Araştırmaları Derneği |
| سنة النشر: |
2017 |
| المجموعة: |
DergiPark Akademik (E-Journals) |
| مصطلحات موضوعية: |
Streptococcus pyogenes,Group A Streptococcus,LAMP PCR,rapiddiagnosis,tonsillopharyngitis, Streptococcus pyogenes,Grup A Streptokok,LAMP PCR,hızlı tanı,tonsillofarenjit |
| الوصف: |
Objective: Most bacterial pharyngitis is caused by Group A Streptococci (GAS). Therefore, discrimination between bacterial-viralcauses, and rapid diagnosis of GAS are important in terms of guiding treatment in acute tonsillopharyngitis cases. Thepresent study aims to assess the effi cacy ofLoop-Mediated Isothermal Amplifi cation PCR (LAMP PCR) test for rapiddiagnosis of GAS, and compare its correlation with culture tests as conventional methods in children with suspectedacute bacterial tonsillopharyngitis.Materials and Methods: We studied 36 GAS strains isolated from swab samples obtained from posterior pharynges and tonsils of patientspresenting to Sakarya University Training and Research Hospital with acute tonsillopharyngitis symptoms, pharyngealswab samples from 8 patients who had concurrent positive pharyngeal culture results for GAS, and one standardStreptococcus pyogenesstrain (ATCC 19615). Isolated GAS strains were analyzed with LAMP PCR. As target region,Streptoplysin B (speB) gene region was selected. Extraction, amplifi cation and analysis of the results were performedwith LAMP PCR analyzer (Genie II, Optigene, UK), and all these procedures were completed within 70 minutes. Resultgraphics obtained from LAMP PCR analyzer were evaluated with Genie Explorer v2.0.6.3 (Optigene, UK) program.Results: LAMP PCR method yielded positive results for 97.2% (35/36) of GAS strains isolated from patient samples. Pharyngealswab samples of 8 patients who had concurrent positive culture results for GAS were analyzed, and 7 of them(87.5%) had positive GAS results with LAMP PCR. Standard strain sample which was analyzed with duplicate run wasalso detected as positive. With enzymatic method, DNA isolation (~30 minutes) and isothermal amplifi cation process(~40 minutes) were completed in ~70 minutes.Conclusion: LAMP PCR has high sensitivity and specifi city with pretty simple and practical extraction procedure. A single smallportable device is suffi cient for isolation, amplifi cation and analysis of the results, with the ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| Relation: |
https://dergipark.org.tr/tr/download/article-file/312748; https://dergipark.org.tr/tr/pub/bshr/issue/29771/320264 |
| الاتاحة: |
https://dergipark.org.tr/tr/pub/bshr/issue/29771/320264 |
| رقم الانضمام: |
edsbas.EBEE3A43 |
| قاعدة البيانات: |
BASE |