التفاصيل البيبلوغرافية
| العنوان: |
Group B Streptococcus cpsE Is Required for Serotype V Capsule Production and Aids in Biofilm Formation and Ascending Infection of the Reproductive Tract during Pregnancy |
| المؤلفون: |
Kristen Noble (10911836), Jacky Lu (9965158), Miriam A. Guevara (9965164), Ryan S. Doster (4670305), Schuyler A. Chambers (9518845), Lisa M. Rogers (1432480), Rebecca E. Moore (9965167), Sabrina K. Spicer (10911839), Alison J. Eastman (10911842), Jamisha D. Francis (9965161), Shannon D. Manning (7406450), Lakshmi Rajagopal (247524), David M. Aronoff (313849), Steven D. Townsend (1903363), Jennifer A. Gaddy (7023797) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Microbiology, Genetics, Infectious Diseases, Computational Biology, Space Science, Biological Sciences not elsewhere classified, group B Streptococcus cpsE, capsule, 1-P, GBS, Serotype V Capsule Production, Pregnancy Group B Streptococcus, cpsE gene product, virulence factor, undecaprenyl lipid molecule, c apsule p olysaccharide, Δ cpsE deletion, encapsulated Gram-positive pathogen, tract |
| الوصف: |
Group B Streptococcus (GBS) is an encapsulated Gram-positive pathogen that causes ascending infections of the reproductive tract during pregnancy. The capsule of this organism is a critical virulence factor that has been implicated in a variety of cellular processes to promote pathogenesis. Primarily comprised of carbohydrates, the GBS capsule and its synthesis is driven by the c apsule p olysaccharide s ynthesis ( cps ) operon. The cpsE gene within this operon encodes a putative glycosyltransferase that is responsible for the transfer of a Glc-1-P from UDP-Glc to an undecaprenyl lipid molecule. We hypothesized that the cpsE gene product is important for GBS virulence and ascending infection during pregnancy. Our work demonstrates that a GBS cpsE mutant secretes fewer carbohydrates, has a reduced capsule, and forms less biofilm than the wild-type parental strain. We show that, compared to the parental strain, the ΔcpsE deletion mutant is more readily taken up by human placental macrophages and has a significantly attenuated ability to invade and proliferate in the mouse reproductive tract. Taken together, these results demonstrate that the cpsE gene product is an important virulence factor that aids in GBS colonization and invasion of the gravid reproductive tract. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Group_B_i_Streptococcus_cpsE_i_Is_Required_for_Serotype_V_Capsule_Production_and_Aids_in_Biofilm_Formation_and_Ascending_Infection_of_the_Reproductive_Tract_during_Pregnancy/14718464 |
| DOI: |
10.1021/acsinfecdis.1c00182.s001 |
| الاتاحة: |
https://doi.org/10.1021/acsinfecdis.1c00182.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.EBB05CF1 |
| قاعدة البيانات: |
BASE |