Academic Journal
Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring
| العنوان: | Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring |
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| المؤلفون: | Zubkovs, Vitalijs, Wang, Hanxuan, Schürgers, Nils, Weninger, Astrid, Glieder, Anton, Cattaneo, Stefano, Boghossian, Ardemis A. |
| المصدر: | ISSN: 2516-0230. |
| سنة النشر: | 2022 |
| المجموعة: | University of Freiburg: FreiDok |
| الوصف: | Single-walled carbon nanotubes (SWCNTs) emit photostable near-infrared (NIR) fluorescence that is conducive for optical glucose monitoring. Such SWCNT-based optical sensors often require the immobilization of proteins that can confer glucose selectivity and reactivity. In this work, we immobilize a glucose-reactive enzyme, glucose oxidase (GOx), onto SWCNTs using a N-(1-pyrenyl)maleimide (PM) crosslinker via thiol bioconjugation of engineered cysteine residues. We compare the conjugation of several glucose oxidase variants containing rationally-engineered cysteines and identify a D70C variant that shows effective bioconjugation. The bioconjugation was characterized through both absorption and fluorescence spectroscopy. Furthermore, we demonstrate an application for continuous glucose monitoring in the NIR-II optical region using the bioconjugated reaction solution, which shows a reversible response to physiological concentrations of glucose. Finally, we develop a miniaturized NIR-II reader to be used for cell cultures that require continuous glucose monitoring. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | English |
| Relation: | https://freidok.uni-freiburg.de/data/244654 |
| DOI: | 10.1039/d2na00092j |
| الاتاحة: | https://freidok.uni-freiburg.de/data/244654 https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-2446549 https://doi.org/10.1039/d2na00092j https://freidok.uni-freiburg.de/dnb/download/244654 |
| Rights: | free |
| رقم الانضمام: | edsbas.EBAA2B9E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1039/d2na00092j |
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