التفاصيل البيبلوغرافية
| العنوان: |
Exposure to various ultraviolet radiations in human skin in situ: Biomarkers for oxidative stress |
| المؤلفون: |
Liardet, S., Schwarzkopf, S., Scaletta, C., Frenk, E., Panizzon, R., Applegate, L.A. |
| المصدر: |
29th Annual Meeting of the European Society for Dermatological Research, vol. 113, pp. 469 |
| سنة النشر: |
1999 |
| المجموعة: |
Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: |
human skin/Oxidative Stress/RADIATION/Skin/Stress/Switzerland |
| الوصف: |
Following UV radiation, reactive oxygen species (ROS) are responsible for the generation of Comet assay (single-cell DNA gel electrophoresis) is widely used in photobiology and toxicology oxidative stress in human skin. ROS are considered to play an important role in developing to determine the degree of DNA damage induced by ultraviolet (UV) radiation or other agents. damage such as photoaging, immune suppression, cataract formation and carcinogenesis. The In principle, DNA fragments produced due to double-strand nicking by various mutagens or putative antioxidant defense system which protects human skin cells against developing such indirectly during DNA repair, are separated by electrophoresis from intact DNA. During singledamage consists of two different types of protective molecules (enzymatic and nonenzymatic cell electrophoresis the DNA fragments form a ''tail'' originating from cell nucleus, producing a pathways). In this manner, we investigated the behavior of two enzymatic radical scavengers, comet-like figure. Usually, the amount of DNA in the tail is measured by image analysis and superoxide dismutase (SOD) and heme oxygenase I (HO-1), in addition to heat shock protein 70 employed to quantify the degree of DNA damage. (Hsp 70) and ferritin following acute irradiation with UVA I, UVA I 1 II, and solar simulating We propose here an alternative method for DNA damage quantification that is based on measuring light. Analysis of the content of these biomarkers in human skin after acute irradiation was of DNA loss from the nucleus. The cells (HaCaT line or human peripheral lymphocytes) are performed by immunohistochemistry on biopsies from previously non-sun exposed sites of prepared and electrophoresed on microscope slides, as in the classic comet assay. DNA is stained individuals with skin type II-III. In general we have found that their induction or depletion seems with propidium iodie, and fluorescence is measured by laser scanning cytometry (LSC) directly to be dependent on cell type, wavelength ... |
| نوع الوثيقة: |
conference object |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/isbn/0022-202X; https://serval.unil.ch/notice/serval:BIB_4985A6F03A3A |
| الاتاحة: |
https://serval.unil.ch/notice/serval:BIB_4985A6F03A3A |
| رقم الانضمام: |
edsbas.EAD948E3 |
| قاعدة البيانات: |
BASE |