Academic Journal
Specificity of binding of the plectin actin-binding domain to beta4
| العنوان: | Specificity of binding of the plectin actin-binding domain to beta4 |
|---|---|
| المؤلفون: | Y H. M. Litjens, Jan Koster, Ingrid Kuikman, Ra Van Wilpe, José M. De Pereda, Arnoud Sonnenberg, Richard Hynes |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.molbiolcell.org/content/14/10/4039.full.pdf. |
| سنة النشر: | 2003 |
| المجموعة: | CiteSeerX |
| الوصف: | Plectin is a major component of the cytoskeleton and links the intermediate filament system to hemidesmosomes by binding to the integrin �4 subunit. Previously, a binding site for �4 was mapped on the actin-binding domain (ABD) of plectin and binding of �4 and F-actin to plectin was shown to be mutually exclusive. Here we show that only the ABDs of plectin and dystonin bind to �4, whereas those of other actin-binding proteins do not. Mutations of the ABD of plectin-1C show that Q131, R138, and N149 are critical for tight binding of the ABD to �4. These residues form a small cavity, occupied by a well-ordered water molecule in the crystal structure. The �4 binding pocket partly overlaps with the actin-binding sequence 2 (ABS2), previously shown to be essential for actin binding. Therefore, steric interference may render binding of �4 and F-actin to plectin mutually exclusive. Finally, we provide evidence indicating that the residues preceding the ABD in plectin-1A and-1C, although unable to mediate binding to �4 themselves, modulate the binding activity of the ABD for �4. These studies demonstrate the unique property of the plectin-ABD to bind to both F-actin and �4, and explain why several other ABD-containing proteins that are expressed in basal keratinocytes are not recruited into hemidesmosomes. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.325.2899; http://www.molbiolcell.org/content/14/10/4039.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.325.2899 http://www.molbiolcell.org/content/14/10/4039.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.EA9DF541 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |