التفاصيل البيبلوغرافية
| العنوان: |
Uptake of glycosylated Ber e 1 and non-glycosylated Ber e 1 and SFA8 by bmDC. |
| المؤلفون: |
Nuzul N. Jambari (10724842), Susan Liddell (144607), Luisa Martinez-Pomares (40302), Marcos J. C. Alcocer (7288967) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Molecular Biology, Biotechnology, Immunology, Cancer, Infectious Diseases, Space Science, RI, rBer e 1, SFA, glycosylated rBer e 1, glycosylation, C-type lectin receptors, allergenic 2 S albumin protein, DC-T cell assay, counterpart, IL -12 production, MR, HLA, yeast Pichia pastoris expression system, murine bone marrow-derived dendriti., Brazil nut allergen, antigenicity, nBer e 1, non-glycosylated nBer e 1, Ser, polarise Th 2 cell response, Ber e 1 |
| الوصف: |
(A.) The contour plots represent cell populations where cells in the gating area are CD11c + bmDC with A488-labelled proteins. DC uptake was performed with increasing concentration (1–50 μg/ml) of A488-labelled 2S albumin proteins for 45 min (B.). Data were normalised as a percentage of A488 + and PE + gated cells relative to the untreated control. (C.) The contour plot showed the distribution of CD11c + /A488 + or FITC + cell population in the gated region. Proteins were internalised as shown when bmDC were pulsed for 45 min with labelled proteins at 4°C (cell-surface bound) and 37°C (internalised). Data were normalised to a percentage of the A488/FITC + bmDC population incubated with Dextran-FITC at 37°C and are presented as mean ±S.D. (n = 2) ****p<0.0001 (D.). |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Uptake_of_glycosylated_Ber_e_1_and_non-glycosylated_Ber_e_1_and_SFA8_by_bmDC_/14513965 |
| DOI: |
10.1371/journal.pone.0249876.g003 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0249876.g003 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.EA6D93E |
| قاعدة البيانات: |
BASE |