Academic Journal
Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius
| العنوان: | Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius |
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| المؤلفون: | Gambelli, Lavinia, McLaren, Mathew, Conners, Rebecca, Sanders, Kelly, Gaines, Matthew C, Clark, Lewis, Gold, Vicki AM, Kattnig, Daniel, Sikora, Mateusz, Hanus, Cyril, Daum, Bertram |
| المساهمون: | Gambelli, Lavinia, McLaren, Mathew, Conners, Rebecca, Sanders, Kelly, Gaines, Matthew C, Clark, Lewis, Gold, Vicki AM, Kattnig, Daniel, Sikora, Mateusz, Hanus, Cyril, Daum, Bertram |
| سنة النشر: | 2024 |
| المجموعة: | Georg-August-Universität Göttingen: GoeScholar |
| الوصف: | Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo electron tomography, and Alphafold2 predictions to generate an atomic model of the two-component S-layer of Sulfolobus acidocaldarius . The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesise that jackknife-like conformational changes in SlaA play important roles in S-layer assembly. ; European Research Council ; Wellcome Trust http://dx.doi.org/10.13039/100010269 ; Wellcome Trust http://dx.doi.org/10.13039/100010269 ; Agence Nationale de la Recherche http://dx.doi.org/10.13039/501100001665 ; Agence Nationale de la Recherche http://dx.doi.org/10.13039/501100001665 ; Leverhulme Trust http://dx.doi.org/10.13039/501100000275 ; Biotechnology and Biological Sciences Research Council http://dx.doi.org/10.13039/501100000268 ; Polish Ministry of Education and Science ; German Federal Ministry of Education and Research |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://resolver.sub.uni-goettingen.de/purl?gro-2/142753 |
| DOI: | 10.7554/eLife.84617 |
| الاتاحة: | https://resolver.sub.uni-goettingen.de/purl?gro-2/142753 https://doi.org/10.7554/eLife.84617 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.EA6A0BB4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/eLife.84617 |
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