Academic Journal
Identification of genes regulated by interleukin-1β in human endometrial stromal cells
| العنوان: | Identification of genes regulated by interleukin-1β in human endometrial stromal cells |
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| المؤلفون: | Rossi, Marco, Sharkey, Andrew M, Viganò, Paola, Fiore, Giovina, Furlong, Rob, Florio, Pasquale, Ambrosini, Guido, Smith, Stephen K, Petraglia, Felice |
| سنة النشر: | 2005 |
| المجموعة: | Istituto Nazionale di Fisica Nucleare (INFN): Open Access Repository |
| مصطلحات موضوعية: | Cell Biology, Obstetrics and Gynecology, Endocrinology, Embryology, Reproductive Medicine |
| الوصف: | Interleukin-1β (IL-1b) is an important immune regulatory factor that in human endometrium plays a role in both menstruation and implantation in the event of pregnancy. It promotes inflammatory-like processes and also stimulates tissue remodelling. We present a cDNA microarray study documenting the major effects of IL-1β on gene expression in stromal cells from human endometrium. Endometrial stromal cells from five normal healthy women at the mid secretory phase were cultured with or without IL-1β at 50 and 500 pg/ml for 48 h. cDNA microarrays were used to compare the levels of gene expression in total RNA isolated from cells stimulated with IL-1β. These cDNA arrays were produced containing 15 164 sequence-verified clones, which included genes known to be important in angiogenesis, immune modulators, apoptosis, cell signalling, extra-cellular matrix (ECM) remodelling and cell cycle regulation. Genes which were regulated by IL-1β were identified by analysis of the microarray data using the Significance Analysis of Microarrays software package. Upregulated (n= 23) and downregulated (n= 6) different genes were observed, which changed at least 3-fold, at a false discovery rate of less than 2% (P< 0.02). Our results have identified genes regulated by IL-1β, which are involved in leukocyte recruitment, ECM remodelling and other cellular functions. Changes in three genes, IL-8, colony-stimulating factor 2 and aldoketo reductase family 1 member 1, which were upregulated by IL-1β, were verified using real-time PCR. Novel functions regulated by IL-1β in endometrium, including genes involved in free radical protection, and fatty acid metabolism were also identified. These results also provide new insights into the role of IL-1β in disorders of the endometrium, especially in implantation-related infertility and endometriosis, in which this cytokine plays a major role. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | url:https://www.openaccessrepository.it/communities/itmirror; https://www.openaccessrepository.it/record/198752 |
| DOI: | 10.1530/rep.1.00688 |
| الاتاحة: | https://www.openaccessrepository.it/record/198752 https://doi.org/10.1530/rep.1.00688 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.EA20FEE2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1530/rep.1.00688 |
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