Academic Journal
Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD
| العنوان: | Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD |
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| المؤلفون: | Da-Ming Huang, Tian-Zhen Zhang, Feng-Jie Cui, Wen-Jing Sun, Li-Ming Zhao, Meng-Yi Yang, Ya-Juan Wang |
| المصدر: | Journal of Biomedicine and Biotechnology, Vol 2011 (2011) |
| بيانات النشر: | Hindawi Limited |
| سنة النشر: | 2011 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65, Medicine |
| الوصف: | A procedure for simultaneous identification and quantification of canrenone and its biotransformed product 11-α-hydroxy-canrenone by high-performance liquid chromatography with ultraviolet detector (HPLC-UVD) and mass spectrometry (LC-MS) methods was proposed. The optimal determination variables on the HPLC-UVD or LC-MS coupled with a ZORBAX Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) were set as follows: detection wavelength of 280 nm, mobile phase of water and methanol gradient elution, temperature for the chromatographic column of 30°C, flow rate of mobile phase of 0.8 mL/min, sample injection volume of 5 μL, and elution time of 40 min. The MS conditions were set as follows: the flow rate of sheath gas, aux gas, and sweep gas were kept at 35 arb, 5 arb, and 0 arb, respectively. The temperature of capillary was held at 300°C, and capillary voltage was set at 30.00 V. Tube lens were performed at 100.00 V. The proposed method was validated by linearity (𝑟2 ≥ 0.9910), average recovery (94.93%, RSD1.21%), precision (RSD ≤ 1.31%), limit of detection, and limit of quantification (LOD 0.1~0.12 mg/L, LOQ 0.5~0.67 mg/L), which proved to be affordable for simultaneously determining canrenone and its bio-transformed product 11-α-hydroxy-canrenone. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1110-7243 1110-7251 |
| Relation: | http://dx.doi.org/10.1155/2011/917232; https://doaj.org/toc/1110-7243; https://doaj.org/toc/1110-7251; https://doaj.org/article/b1fe37c235924cd093553a36dd033450 |
| DOI: | 10.1155/2011/917232 |
| الاتاحة: | https://doi.org/10.1155/2011/917232 https://doaj.org/article/b1fe37c235924cd093553a36dd033450 |
| رقم الانضمام: | edsbas.E9F4EF1 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 11107243 11107251 |
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| DOI: | 10.1155/2011/917232 |