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PGT145 does not protect against wild-type SIV mac 239 challenge.
| العنوان: | PGT145 does not protect against wild-type SIV mac 239 challenge. |
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| المؤلفون: | Michael W. Grunst, Hwi Min Gil, Andres G. Grandea III, Brian J. Snow, Raiees Andrabi, Rebecca Nedellec, Iszac Burton, Natasha M. Clark, Sanath Kumar Janaka, Nida K. Keles, Ryan V. Moriarty, Andrea M. Weiler, Saverio Capuano III, Christine M. Fennessey, Thomas C. Friedrich, Shelby L. O’Connor, David H. O’Connor, Aimee T. Broman, Brandon F. Keele, Jeffrey D. Lifson, Lars Hangartner, Dennis R. Burton, David T. Evans |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Cell Biology, Molecular Biology, Biotechnology, Evolutionary Biology, Immunology, Cancer, Mental Health, Infectious Diseases, Plant Biology, Virology, despite high concentrations, dependent cellular cytotoxicity, two additional groups, animals became infected, treated animals compared, div >< p, treated control animals, six rhesus macaques, potent adcc activity, increases pgt145 binding, 1 env trimer, treated animals, six macaques, separate groups, infected cells, 1 replication, potent antibody, control antibody |
| الوصف: | (A) ADCC responses in plasma at the time of challenge. CEM. NKR- CCR5-sLTR-Luc cells infected with SIV mac 239 were incubated with a NK cell line expressing rhesus macaque CD16 at a 10:1 effector/target ratio in the presence of serial dilutions of plasma collected on the day of challenge from animals treated with either PGT145 or DEN3. (B) Neutralization activity in plasma at the time of challenge. ADCC was calculated from the dose-dependent loss of luciferase activity (% RLU) after an 8 hour incubation. SIV mac 239 was incubated in the presence of serial dilutions of plasma for 1 hour before addition to TZM-bl cells. Luciferase induction was measured after 48 hours and neutralization was calculated from the dose-dependent inhibition of luciferase (% RLU). (C) Post-challenge viral loads. Viral RNA loads in plasma were measured using a qRT-PCR assay with a detection threshold of 15 copies/ml. Geometric mean viral loads for the DEN3 and PGT145 groups are shown as dashed lines. Peak and chronic phase (8–20 weeks post-challenge) viral loads were not significantly different in the animals treated with PGT145 and DEN (p = 0.4, SE = 0.20, linear regression and p = 0.4, SE = 0.46, linear mixed models, respectively). |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/PGT145_does_not_protect_against_wild-type_SIV_sub_mac_sub_239_challenge_/25043373 |
| DOI: | 10.1371/journal.ppat.1011819.g002 |
| الاتاحة: | https://doi.org/10.1371/journal.ppat.1011819.g002 https://figshare.com/articles/figure/PGT145_does_not_protect_against_wild-type_SIV_sub_mac_sub_239_challenge_/25043373 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.E9C6FE4A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.ppat.1011819.g002 |
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