Academic Journal
Detection of isoniazid and rifampin resistance of Mycobacterium tuberculosis by a multiplex allele-specific polymerase chain reaction (PCR) assay
| العنوان: | Detection of isoniazid and rifampin resistance of Mycobacterium tuberculosis by a multiplex allele-specific polymerase chain reaction (PCR) assay |
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| المؤلفون: | Zeyneb Allegui, Asma Ghariani, Henda Draoui, Ameni Ghezal, Emna Mehiri, Leila Slim-Saidi |
| المصدر: | International Journal of Mycobacteriology, Vol 1, Iss 1, Pp 34-39 (2012) |
| بيانات النشر: | Wolters Kluwer Medknow Publications |
| سنة النشر: | 2012 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Multidrug-resistant, Tuberculosis, Multiplex PCR, Mutations, Microbiology, QR1-502 |
| الوصف: | Background: The use of molecular techniques is a major improvement for the rapid routine detection and control of multidrug-resistant tuberculosis (MDR-TB). Materials: In this study, the multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to simultaneously detect the most frequent mutations associated with isoniazid (INH) and rifampin (RIF) resistance in a single assay. Results: The assay was tested with 53 clinical isolates. Among them, 27 were MDR strains, 17 were mono-resistant to INH, one was mono-resistant to RIF, and eight were susceptible. The MAS-PCR assay showed a specificity of 100% in detecting drug resistance. An equivalent sensitivity of 92.6% in detecting MDR and RIF-resistance was found. The sensitivity for the detection of INH-resistance was 88.6%. Conclusions: The MAS-PCR assay was a simple and rapid method for detecting the INH and RIF-resistance in Mycobacterium tuberculosis (MT) clinical strains. It is also easy to perform and to interpret. The assay is inexpensive and a less-demanding technique. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2212-5531 2212-554X |
| Relation: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2012;volume=1;issue=1;spage=34;epage=39;aulast=Allegui; https://doaj.org/toc/2212-5531; https://doaj.org/toc/2212-554X; https://doaj.org/article/ebc3e3bb5b0d43b295777d67c11770ab |
| DOI: | 10.1016/j.ijmyco.2012.01.006 |
| الاتاحة: | https://doi.org/10.1016/j.ijmyco.2012.01.006 https://doaj.org/article/ebc3e3bb5b0d43b295777d67c11770ab |
| رقم الانضمام: | edsbas.E9C64385 |
| قاعدة البيانات: | BASE |
| تدمد: | 22125531 2212554X |
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| DOI: | 10.1016/j.ijmyco.2012.01.006 |