Academic Journal
Radiolabelling an F-18 biologic via facile IEDDA 'click' chemistry on the GE FASTLab (TM) platform
| العنوان: | Radiolabelling an F-18 biologic via facile IEDDA 'click' chemistry on the GE FASTLab (TM) platform |
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| المؤلفون: | Allott, L, Amgheib, A, Barnes, C, Braga, M, Brickute, D, Wang, N, Fu, R, Ghaem-Maghami, S, Aboagye, EO |
| المساهمون: | Imperial College Healthcare NHS Trust- BRC Funding, Imperial College Healthcare NHS Trust |
| المصدر: | 1078 ; 1070 |
| بيانات النشر: | Royal Society of Chemistry |
| سنة النشر: | 2021 |
| المجموعة: | Imperial College London: Spiral |
| مصطلحات موضوعية: | Science & Technology, Physical Sciences, Technology, Chemistry, Multidisciplinary, Engineering, Chemical |
| الوصف: | The use of biologics in positron emission tomography (PET) imaging is an important area of radiopharmaceutical development and new automated methods are required to facilitate their production. We report an automated radiosynthesis method to produce a radiolabelled biologic via facile inverse electron demand Diels–Alder (IEDDA) “click” chemistry on a single GE FASTLab™ cassette. We exemplified the method by producing a fluorine-18 radiolabelled interleukin-2 (IL2) radioconjugate from a trans-cyclooctene (TCO) modified IL2 precursor. The radioconjugate was produced using a fully automated radiosynthesis on a single FASTLab™ cassette in a decay-corrected radiochemical yield (RCY, d.c.) of 19.8 ± 2.6% in 110 min (from start of synthesis); the molar activity was 132.3 ± 14.6 GBq μmol−1. The in vitro uptake of [18F]TTCO-IL2 correlated with the differential receptor expression (CD25, CD122, CD132) in PC3, NK-92 and activated human PBMCs. The automated method may be adapted for the radiosynthesis of any TCO-modified protein via IEDDA chemistry. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2058-9883 |
| Relation: | Reaction Chemistry and Engineering; http://hdl.handle.net/10044/1/89785; RDC04; RDB22 79560 |
| DOI: | 10.1039/d1re00117e |
| الاتاحة: | http://hdl.handle.net/10044/1/89785 https://doi.org/10.1039/d1re00117e |
| Rights: | © The Royal Society of Chemistry 2021. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. ; http://creativecommons.org/licenses/by/3.0/ |
| رقم الانضمام: | edsbas.E9142A3F |
| قاعدة البيانات: | BASE |
| تدمد: | 20589883 |
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| DOI: | 10.1039/d1re00117e |