التفاصيل البيبلوغرافية
| العنوان: |
In vivo and in vitro characterization of the canine nephron and urinary proteome |
| المؤلفون: |
Brandt, Laura Elizabeth, author, Bohn, Andrea A., advisor, Ehrhart, Eugene J., committee member, Olver, Christine S., committee member, Prenni, Jessica E., committee member |
| بيانات النشر: |
Colorado State University. Libraries |
| سنة النشر: |
2007 |
| المجموعة: |
Digital Collections of Colorado (Colorado State University) |
| مصطلحات موضوعية: |
biomarkers, canine, immunohistochemistry, nephron, renal failure, urinary proteome |
| الوصف: |
2011 Spring. ; Includes bibliographical references. ; Immunohistochemistry allows the localization of proteins to specific regions of the nephron. This study reports the identification and localization of proteins in situ within normal canine, feline and mouse kidney by immunohistochemistry, maps their distribution and compares results to previously reported findings in other species. The proteins investigated are aquaporin-1, aquaporin-2, calbindin-d28k, glutathione S-transferase-α and Tamm-Horsfall protein. Our findings in the dog are similar to that in other species, and localize aquaporin-1 to the proximal convoluted tubule epithelium, vasa recta endothelium and descending thin limbs, aquaporin-2 to collecting duct epithelium and calbindin-d28k is found within distal convoluted tubule epithelium. Glutathione S-transferase-α has variable expression and is found in renal transitional epithelium only in some individuals, the proximal straight tubules only in some individuals or in both locations in others. Tamm-Horsfall protein localizes to thick ascending limb epithelium. These findings are similar in the cat, with the exception that aquaporin-1 is located in glomerular podocytes, in addition to proximal convoluted tubule epithelium, and glutathione S-transferase-α is found solely within the proximal convoluted tubule within all kidney samples examined. The mouse kidney is almost identical to the dog, but expresses glutathione S-transferase-α in the glomeruli only. Additionally, we successfully adapted techniques employed in human studies to characterize the normal canine urinary proteome. Both soluble proteins and the exosomal fraction of urine were examined. Greater than 500 proteins were identified in this initial study. Many of these proteins are also found in human urine, but large numbers of proteins also appear to be unique to the canine. Software entitled Biological Networks Gene Ontology (BiNGO) was utilized to characterize canine urinary proteins into respective Gene Ontology categories of Cellular ... |
| نوع الوثيقة: |
text |
| وصف الملف: |
born digital; masters theses; application/pdf |
| اللغة: |
English |
| Relation: |
Brandt_colostate_0053N_10435.pdf; http://hdl.handle.net/10217/47306 |
| الاتاحة: |
http://hdl.handle.net/10217/47306 |
| Rights: |
Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. |
| رقم الانضمام: |
edsbas.E8EACB87 |
| قاعدة البيانات: |
BASE |