Academic Journal
Subtracted Transcriptome Profile of Tiger Shrimp ( Penaeus monodon) That Survived WSSV Challenge
| العنوان: | Subtracted Transcriptome Profile of Tiger Shrimp ( Penaeus monodon) That Survived WSSV Challenge |
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| المؤلفون: | Maralit, Benedict A., Ventolero, Minerva Fatimae H., Maningas, Mary Beth B., Amar, Edgar C., Santos, Mudjekeewis D. |
| المساهمون: | Hannoufa, A., Okazaki, Y., Padhukasahasram, B. |
| المصدر: | Dataset Papers in Science ; volume 2014, issue 1 ; ISSN 2314-8497 2314-8497 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2014 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | There is increased interest in the development of virus‐resistant or improved shrimp stock because production is currently hindered by outbreaks and limited understanding of shrimp defense. Recent advancement now allows for high‐throughput molecular studies on shrimp immunity. We used next‐generation sequencing (NGS) coupled with suppression subtractive hybridization (SSH) to generate a transcriptome database of genes from tiger shrimp that survived White spot syndrome virus (WSSV) challenge. A total of 9,597 unique sequences were uploaded to NCBI Sequence Read Archive with accession number SRR577080. Sixty‐five unique sequences, 6% of the total, were homologous to genes of Penaeus monodon . Genes that were initially related to bacterial infection and environmental stress such as 14‐3‐3 gene, heat shock protein 90, and calreticulin were also found including a few full‐length gene sequences. Initial analysis of the expression of some genes was done. Hemocyanin, ferritin, and fortilin‐binding protein exhibited differential expression between survivor and control tiger shrimps. Furthermore, candidate microsatellite markers for brood stock selection were mined and tested. Four trinucleotide and one dinucleotide microsatellites were successfully amplified. The study highlights the advantage of the NGS platform coupled with SSH in terms of gene discovery and marker generation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1155/2014/807806 |
| الاتاحة: | http://dx.doi.org/10.1155/2014/807806 http://downloads.hindawi.com/archive/2014/807806.pdf http://downloads.hindawi.com/archive/2014/807806.xml https://onlinelibrary.wiley.com/doi/pdf/10.1155/2014/807806 |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| رقم الانضمام: | edsbas.E8DC2BA4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/2014/807806 |
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