التفاصيل البيبلوغرافية
| العنوان: |
CUL9 KO cells have significantly altered levels of key proteins in metabolism as determined by iTRAQ. |
| المؤلفون: |
Natalya A. Ortolano (10291721), Alejandra I. Romero-Morales (10291724), Megan L. Rasmussen (10291727), Caroline Bodnya (10291730), Leigh A. Kline (10291733), Piyush Joshi (6816557), Jon P. Connelly (10291736), Kristie L. Rose (744759), Shondra M. Pruett-Miller (10291739), Vivian Gama (10291742) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Developmental Biology, Cancer, Biological Sciences not elsewhere classified, CUL 9 protein levels, CUL 9, Cullin RING ligases, cell models CUL 9, APC, transcriptional regulation pathways, CRL, E 3 ubiquitin ligases, cell cycle regulator, ATP, CUL 9 substrates, FZR, Quantitative proteomic analysis, LC-MS, oxygen consumption rates, CUL 9 KO hPSCs, CUL 9 deletion, cell cycle progression, CUL 9 protein causes |
| الوصف: |
( A ) Protocol for iTRAQ labeling of samples. Two comparisons were done: (1) iPSC WT, iPSC CUL9 KO, NSC WT, and NSC CUL9 KO. (2) NSC WT, NSC CUL9 KO, NPC WT, and NPC CUL9 KO. Each comparison was done for CUL9 KO #1 (n = 1) and #2 (n = 1). ( B ) Venn diagram showing number of significantly altered proteins in iPSC iTRAQ data and hNPC data. Two proteins were significantly altered in both datasets. ( C ) Results from query using Search Tool for the retrieval of Interacting Genes/Proteins (STRING). Diagram depicts known and predicted direct and indirect protein-protein interactions amongst the significant proteins identified in CUL9 KO hPSCs and NPCs. Proteins displayed are significantly (B-H method) altered in one clone and have an average normalized fold change of ≥1.2 or ≤ 0.8 in the other. Colored nodes represent queried proteins and the first shell of interactors. Clear nodes indicate second shell of interactors. Filled nodes show known, partial, or predicted structure. Empty nodes indicate non known or predicted structure is available for the given protein. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/CUL9_KO_cells_have_significantly_altered_levels_of_key_proteins_in_metabolism_as_determined_by_iTRAQ_/14202968 |
| DOI: |
10.1371/journal.pone.0248000.g007 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0248000.g007 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.E8CEDBEE |
| قاعدة البيانات: |
BASE |