Academic Journal
West African agamas: systematics, geographic distribution, ecology and phylogeny
| العنوان: | West African agamas: systematics, geographic distribution, ecology and phylogeny |
|---|---|
| المؤلفون: | MEDIANNIKOV O., TRAPE S., MANE Y., TRAPE J.-F. |
| بيانات النشر: | Федеральное государственное бюджетное образовательное учреждение высшего образования «Саратовский национальный исследовательский государственный университет имени Н. Г. Чернышевского» |
| سنة النشر: | 2010 |
| المجموعة: | CyberLeninka (Scientific Electronic Library) / Научная электронная библиотека «Киберленинка» |
| مصطلحات موضوعية: | AGAMA,SYSTEMATICS,GEOGRAPHIC DISTRIBUTION,ECOLOGY AND PHYLOGENY,WEST AFRICA |
| الوصف: | Few published data are available on West African Agamid lizards. From 2003 to 2009, we have collected 1,020 specimens from 11 countries (Senegal, Mauritania, Mali, Guinea, Liberia, Burkina Faso, Ghana, Togo, Benin, Niger, Nigeria) and investigated their systematics, geographic distribution, ecology and phylogeny. For molecular studies, we have used two separate portions of mitogenome, coding for 16S rRNA and for cytochrome B. DNA from 100 mg of homogenized muscular tissue conserved in ethanol, was extracted using the QIAGEN BioRobot MDx Workstation with customized extraction protocol and stored at 4°C until use in PCR amplifications. Primers were manufactured by Eurogentec, Seraing, Belgium. Polymerase chain reactions were performed in automated DNA thermal cyclers. PCR products were visualized by electrophoresis on a 1.5% agarose gel, stained with ethidium bromide and examined using an ultraviolet transilluminator. The PCR products were purified using a QIAquick Spin PCR Purification Kit (Qiagen) according to the manufacturer's instructions. Sequencing of amplicons was performed using the BigDye Terminator Cycle Sequencing Kit with ABI automated sequencer. Obtained sequences were assembled, edited by BioEdit Sequence alignment editor v. 7.0.9.0 and compared with those available in GenBank by NCBI BLAST. Sequences of both mitochondrial genes from studied lizards were concatenated and aligned with CLUSTAL W program, and a neighbor-joining phylogenetic tree was constructed with Geneious 4.7.6 software. Fourteen different species were recognized in the genus Agama in West Africa, excluding the easternmost part of Nigeria near the Cameroon border which was not included in our study: Agama africana, A. agama, A. boueti, A. boulengeri, A. castroviejoi, A. cristata, A. doriae benueensis, A. gracilimembris, A. insularis, A. paragama, A. sanka-ranica, A. weidholzi, and two undescribed species. According to biogeographic areas, four species were Sahelian, seven species were Sudanian, two species were Guinean, and one ... |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | unknown |
| الاتاحة: | http://cyberleninka.ru/article/n/west-african-agamas-systematics-geographic-distribution-ecology-and-phylogeny http://cyberleninka.ru/article_covers/16502539.png |
| رقم الانضمام: | edsbas.E8BE31EA |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |