Academic Journal
Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture
| العنوان: | Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture |
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| المؤلفون: | Foulon, Mélanie, Marion, Estelle, Marsollier, Laurent |
| المساهمون: | Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers (CRCI2NA), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) |
| المصدر: | ISSN: 1064-3745. |
| بيانات النشر: | HAL CCSD Humana Press/Springer Imprint |
| سنة النشر: | 2022 |
| المجموعة: | Université de Nantes: HAL-UNIV-NANTES |
| مصطلحات موضوعية: | Differential centrifugation, Extracellular vesicles, Mycolactone, Nano-tracking, MESH: Bacterial Toxins, MESH: Buruli Ulcer, MESH: Extracellular Vesicles, MESH: Humans, MESH: Macrolides, MESH: Mycobacterium Infections, MESH: Mycobacterium ulcerans, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience ; Extracellular vesicles (EVs) from both eukaryotic and prokaryotic cells have been characterized over decades and present many biological properties. Since it has been shown that mycobacterial extracellular vesicles (MEVs) of M. ulcerans contain the macrolide toxin mycolactone, MEVs are known to be associated with the pathogenesis of mycobacteria. This chapter describes a method for purifying and characterizing vesicles from in vitro cultures of M. ulcerans. We also describe how purified vesicles can be used in cellular tests, to determine their role in the pathophysiology of M. ulcerans infection. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34643900; PUBMED: 34643900 |
| DOI: | 10.1007/978-1-0716-1779-3_5 |
| الاتاحة: | https://nantes-universite.hal.science/hal-04759652 https://doi.org/10.1007/978-1-0716-1779-3_5 |
| رقم الانضمام: | edsbas.E8B41529 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-0716-1779-3_5 |
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