Academic Journal
Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs
| العنوان: | Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs |
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| المؤلفون: | Tang, Guiliang, Yan, Jun, Gu, Yiyou, Qiao, Mengmeng, Fan, Ruiwen, Mao, Yiping, Tang, Xiaoqing |
| المصدر: | Michigan Tech Publications |
| بيانات النشر: | Digital Commons @ Michigan Tech |
| سنة النشر: | 2012 |
| المجموعة: | Michigan Technological University: Digital Commons @ Michigan Tech |
| مصطلحات موضوعية: | CeRNA, MiR-30d, MiR165/166, MiRNA, MiRNA sponge, Short tandem target mimic (STTM), Small RNA, Target mimic |
| الوصف: | Small RNAs are widespread in plants and animals. They largely include microRNAs (miRNAs) and short interfering RNAs (siRNAs), and they play key roles in gene and chromatin regulations. Here we describe in detail the method for an effective construction of the recently developed short tandem target mimic (STTM) technology to block small RNA functions in plants and animals. STTM is a powerful technology complementing the previous target mimic (TM) in plants and the miRNA sponge, as well as the recently defined endogenous competing RNA (CeRNA) in animals. We expect STTM will not only be effective in blocking small RNA functions in plants but will also become a popular approach in animals. © 2012 Elsevier Inc. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| Relation: | https://digitalcommons.mtu.edu/michigantech-p/7186; https://doi.org/10.1016/j.ymeth.2012.10.006 |
| DOI: | 10.1016/j.ymeth.2012.10.006 |
| الاتاحة: | https://digitalcommons.mtu.edu/michigantech-p/7186 https://doi.org/10.1016/j.ymeth.2012.10.006 |
| رقم الانضمام: | edsbas.E89C755B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ymeth.2012.10.006 |
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