Academic Journal
Expression of adenosine A 2A receptors in the rat lumbar spinal cord and implications in the modulation of N-methyl-d-aspartate receptor currents.
| العنوان: | Expression of adenosine A 2A receptors in the rat lumbar spinal cord and implications in the modulation of N-methyl-d-aspartate receptor currents. |
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| المؤلفون: | Guntz, Emmanuel, Dumont, Hélène, Pastijn, Els, de Kerchove d'Exaerde, Alban, Azdad, Karima, Sosnowski, Maurice, Schiffmann, Serge N., Gall, David |
| المصدر: | Anesthesia and analgesia, 106 (6 |
| سنة النشر: | 2008 |
| المجموعة: | DI-fusion : dépôt institutionnel de l'Université libre de Bruxelles (ULB) |
| مصطلحات موضوعية: | Sciences bio-médicales et agricoles, Adenosine -- analogs & derivatives, Adenosine -- pharmacology, Animals, Blotting, Western, Glycine -- metabolism, Lumbosacral Region, Membrane Potentials, N-Methylaspartate -- metabolism, Pain -- metabolism, Patch-Clamp Techniques, Phenethylamines -- pharmacology, Posterior Horn Cells -- drug effects, Posterior Horn Cells -- metabolism, RNA, Messenger -- analysis, Rats, Wistar, Receptor, Adenosine A2A -- agonists, Adenosine A2A -- genetics, Adenosine A2A -- metabolism, Receptors, N-Methyl-D-Aspartate -- antagonists & inhibitors, N-Methyl-D-Aspartate -- metabolism, Reverse Transcriptase Polymerase Chain Reaction, Spinal Cord -- drug effects, Spinal Cord -- metabolism, Substantia Gelatinosa -- metabolism |
| الوصف: | BACKGROUND: The presence of A(2A) receptors in the dorsal horn of the spinal cord remains controversial. At this level, activation of N-methyl-d-aspartate (NMDA) receptors induces wind-up, which is clinically expressed as hyperalgesia. Inhibition of NMDA receptor currents after activation of A(2A) receptors has been shown in rat neostriatal neurons. In this study, we sought to establish the presence of adenosine A(2A) receptors in the lamina II of the rat lumbar dorsal horn neurons and investigated whether the activation of A(2A) receptors is able to modulate NMDA receptor currents. METHODS: Experiments were conducted in the rat lumbar spinal cord. The presence of adenosine A(2A) receptor transcripts inside the lumbar spinal cord is assessed with the reverse transcriptase polymerase chain reaction (RT-PCR) technique. Western blot experiments are performed at the same level. The RT-PCR technique is also performed specifically in the lamina II, and the presence of adenosine A(2A) receptor transcripts is assessed in neurons from the lamina II with the single-cell RT-PCR technique. The effect of adenosine A(2A) receptor activation on NMDA receptor currents is studied by the whole-cell configuration of the patch clamp technique. RESULTS: RT-PCR performed on the lumbar spinal cord revealed the presence of adenosine A(2A) receptor transcripts. Western blot experiments revealed the presence of A(2A) receptors in the lumbar spinal cord. RT-PCR performed on the substantia gelatinosa also revealed the presence of adenosine A(2A) receptor transcripts. Finally, single cell RT-PCR revealed the presence of adenosine A(2A) receptor transcripts in a sample of lamina II neurons. Patch clamp recordings showed an inhibition of NMDA currents during the application of a selective A(2A) agonist. CONCLUSIONS: These results demonstrate the presence of A(2A) receptor on neurons from the substantia gelatinosa of the rat lumbar dorsal horn and the inhibition of NMDA-induced currents by the application of a selective A(2A) receptor agonist. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 1 full-text file(s): application/pdf |
| اللغة: | English |
| Relation: | uri/info:doi/10.1213/ane.0b013e318173251f; uri/info:pii/106/6/1882; uri/info:pmid/18499627; uri/info:scp/44649145877; https://dipot.ulb.ac.be/dspace/bitstream/2013/52074/1/AnestAnalg2008A2A.pdf; http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/52074 |
| الاتاحة: | http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/52074 https://dipot.ulb.ac.be/dspace/bitstream/2013/52074/1/AnestAnalg2008A2A.pdf |
| رقم الانضمام: | edsbas.E867EFF3 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |