Academic Journal
Xenon preconditioning: the role of prosurvival signaling, mitochondrial permeability transition and bioenergetics in rats.
| العنوان: | Xenon preconditioning: the role of prosurvival signaling, mitochondrial permeability transition and bioenergetics in rats. |
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| المساهمون: | Yasushi Mio, Yon Hee shim, Ebony Richards, Zeljko J. Bosnjak, Paul S. Pagel, Martin Bienengraeber, Shim, Yon Hee |
| المصدر: | T200902955.pdf |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Anesthetics, Inhalation/pharmacology, Animals, Blood Pressure/drug effects, Blotting, Western, Calcium/pharmacology, Cardiotonic Agents, Energy Metabolism/drug effects, Glycogen Synthase Kinase 3/metabolism, Glycogen Synthase Kinase 3 beta, Heart Rate/drug effects, Ischemic Preconditioning, Myocardial, Male, Mitochondria, Heart/drug effects, Heart/pathology, Myocardial Infarction/pathology, Myocardial Infarction/prevention & control, Myocardial Reperfusion Injury/pathology, Myocardial Reperfusion Injury/prevention & control, Oxygen Consumption/drug effects, Permeability/drug effects, Phosphorylation, Proto-Oncogene Proteins c-akt/metabolism, Rats, Wistar, Xenon/pharmacology |
| الوصف: | BACKGROUND: Similar to volatile anesthetics, the anesthetic noble gas xenon protects the heart from ischemia/reperfusion injury, but the mechanisms responsible for this phenomenon are not fully understood. We tested the hypothesis that xenon-induced cardioprotection is mediated by prosurvival signaling kinases that target mitochondria. METHODS: Male Wistar rats instrumented for hemodynamic measurements were subjected to a 30 min left anterior descending coronary artery occlusion and 2 h reperfusion. Rats were randomly assigned to receive 70% nitrogen/30% oxygen (control) or three 5-min cycles of 70% xenon/30% oxygen interspersed with the oxygen/nitrogen mixture administered for 5 min followed by a 15 min memory period. Myocardial infarct size was measured using triphenyltetrazolium staining. Additional hearts from control and xenon-pretreated rats were excised for Western blotting of Akt and glycogen synthase kinase 3 beta (GSK-3beta) phosphorylation and isolation of mitochondria. Mitochondrial oxygen consumption before and after hypoxia/reoxygenation and mitochondrial permeability transition pore opening were determined. RESULTS: Xenon significantly (P < 0.05) reduced myocardial infarct size compared with control (32 +/- 4 and 59% +/- 4% of the left ventricular area at risk; mean +/- sd) and enhanced phosphorylation of Akt and GSK-3beta. Xenon pretreatment preserved state 3 respiration of isolated mitochondria compared with the results obtained in the absence of the gas. The Ca(2+) concentration required to induce mitochondrial membrane depolarization was larger in the presence compared with the absence of xenon pretreatment (78 +/- 17 and 56 +/- 17 microM, respectively). The phosphoinositol-3-kinase-kinase inhibitor wortmannin blocked the effect of xenon on infarct size and respiration. CONCLUSIONS: These results indicate that xenon preconditioning reduces myocardial infarct size, phosphorylates Akt, and GSK-3beta, preserves mitochondrial function, and inhibits Ca(2+)-induced mitochondrial permeability ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 858~866 |
| اللغة: | unknown |
| تدمد: | 0003-2999 1526-7598 |
| Relation: | ANESTHESIA AND ANALGESIA; J00144; OAK-2009-01163; https://ir.ymlib.yonsei.ac.kr/handle/22282913/104503; T200902955; ANESTHESIA AND ANALGESIA, Vol.108(3) : 858-866, 2009 |
| DOI: | 10.1213/ane.0b013e318192a520 |
| الاتاحة: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104503 https://doi.org/10.1213/ane.0b013e318192a520 |
| Rights: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ |
| رقم الانضمام: | edsbas.E835292E |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00032999 15267598 |
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| DOI: | 10.1213/ane.0b013e318192a520 |