Academic Journal

Induction of β -1,3-glucanase in barley in response to infection by fungal pathogens

التفاصيل البيبلوغرافية
العنوان: Induction of β -1,3-glucanase in barley in response to infection by fungal pathogens
المؤلفون: Jutidamrongphan, W., Andersen, J. B., Mackinnon, G., Manners, J. M., Simpson, R. S., Scott, K. J.
سنة النشر: 1991
المجموعة: The University of Queensland: UQ eSpace
مصطلحات موضوعية: Disease resistance, 1102 Agronomy and Crop Science, 1314 Physiology
الوصف: The sequence of a partial cDNA clone corresponding to an mRNA induced in leaves of barley (Hordeum vulgare) by infection with fungal pathogens matched almost perfectly with that of a cDNA clone coding for β-1,3-glucanase isolated from the scutellum of barley. Western blot analysis of intercellular proteins from near-isogenic barley lines inoculated with the powdery mildew fungus (Erysiphe graminis f. sp. hordei) showed a strong induction of glucanase in all inoculated lines but was most pronounced in two resistant lines. These data were confirmed by β -1,3-glucanase assays. The barley cDNA was used as a hybridization probe to detect mRNAs in barley, wheat (Triticum aestivum), rice (Oryza sativus), and sorghum (Sorghum bicolor), which are induced by infection with the necrotrophic pathogen Bipolaris sorokiniana. These results demonstrate that activation of β -1,3-glucanase genes may be a general response of cereals to infection by fungal pathogens.
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 0894-0282
الاتاحة: https://espace.library.uq.edu.au/view/UQ:707634
رقم الانضمام: edsbas.E7FCCC69
قاعدة البيانات: BASE