Academic Journal
Effects of exogenous agmatine in human leukemia HMC-1 and HL-60 cells on prolifertation, polyamine metabolism and cell cycle
| العنوان: | Effects of exogenous agmatine in human leukemia HMC-1 and HL-60 cells on prolifertation, polyamine metabolism and cell cycle |
|---|---|
| المؤلفون: | Haenisch, B., Bönisch, H., Cichon, S., Allam, J.P., Novak, N., Molderings, G.J. |
| المصدر: | Leukemia research 35, 1248 - 1253 (2011). doi:10.1016/j.leukres.2010.12.023 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2011 |
| المجموعة: | Forschungszentrum Jülich: JuSER (Juelich Shared Electronic Resources) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/610, Agmatine: pharmacology, Agmatine: therapeutic use, Antineoplastic Agents: pharmacology, Antineoplastic Agents: therapeutic use, Cell Cycle: drug effects, Cell Line, Tumor, Cell Proliferation: drug effects, Drug Evaluation, Preclinical, Gene Expression Profiling, Gene Expression Regulation, Leukemic: drug effects, HL-60 Cells, Humans, Leukemia: drug therapy, Leukemia: genetics, Leukemia: pathology, Microarray Analysis, Polyamines: metabolism, Antineoplastic Agents, Polyamines, Agmatine, HMC-1, HL-60, Leukemia, Polyamine pathway, Genome-wide gene expression profiling |
| جغرافية الموضوع: | DE |
| الوصف: | Impairment of agmatine homeostasis is involved in the regulation of cell proliferation in malignant solid tumors. The present study aimed at analyzing the relevance of agmatine homeostasis in pathophysiology of human leukemia. Proliferation of the human leukemia cells HMC-1 and HL-60 was determined in the absence or presence of agmatine. Apoptosis and cell cycle distribution was investigated by determination of caspase-3 activity and/or flow cytometry after staining with propidium iodide. Expression analysis was performed by qPCR and by a microarray genechip. Exogenous agmatine inhibited proliferation of both HMC-1 and HL-60 cells. The antiproliferative effect was due to interference of agmatine with the cell cycle with no evident signs of apoptosis. Comparative analysis of expression of mRNA in untreated HMC-1 cells and in non-leukemic human mast cells revealed a much lower expression of agmatinase and diamine oxidase in HMC-1 cells indicating a significantly reduced agmatine catabolism in the leukemic cells. At the mRNA level, inhibition of proliferation of both cell lines by agmatine was associated with cell type-specific alterations of the expression of enzymes of the polyamine pathway. The present results point to a significant role of agmatine homeostasis in the (patho)physiology of cell proliferation of leukemic cells, at least in HMC-1 and HL-60 cells, that may serve as a potential target for an adjuvant therapy in the treatment of human leukemia. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/pmid:21236489; info:eu-repo/semantics/altIdentifier/wos/WOS:000293778200038; info:eu-repo/semantics/altIdentifier/issn/0145-2126; https://juser.fz-juelich.de/record/17363; https://juser.fz-juelich.de/search?p=id:%22PreJuSER-17363%22 |
| الاتاحة: | https://juser.fz-juelich.de/record/17363 https://juser.fz-juelich.de/search?p=id:%22PreJuSER-17363%22 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.E7D3C50C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |