التفاصيل البيبلوغرافية
| العنوان: |
Construction and Characterization of a Gradient Strength Promoter Library for Fine-Tuned Gene Expression in Bacillus licheniformis |
| المؤلفون: |
Yi Rao (469255), Peifen Li (5231681), Xinxin Xie (2104246), Jiemin Li (9421540), Yongqing Liao (11349407), Xin Ma (37268), Dongbo Cai (3948317), Shouwen Chen (3550844) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Genetics, Molecular Biology, Physiology, Pharmacology, Biotechnology, Ecology, Developmental Biology, Science Policy, Plant Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, via manipulating synthetic, red fluorescent protein, pucheriminic acid yield, pucheriminic acid synthesis, original strain dw2, important industrial strain, green fluorescent protein, 6 – 741, tuning gene expression, tuned gene expression, keratinase expression assays, gene expression hindered, undh , ubay , baca , bacillus licheniformis dw2, bacillus licheniformis <, strong ) |
| الوصف: |
Bacillus licheniformis DW2 is an important industrial strain for bacitracin production, and it is also used for biochemical production, however, the lack of effective toolkit for precise regulation of gene expression hindered its application seriously. Here, a gradient strength promoter library was constructed based on bacitracin synthetase gene cluster promoter P bacA . First, different P bacA promoter variants were constructed via coupling P bacA with various 5′-UTRs, and expression ranges of 32.6–741.8% were attained among these promoters. Then, three promoters, P Ubay (strong), P bacA (middle), and P Undh (weakest), were applied for red fluorescent protein (RFP) and keratinase expression assays, and these promoters were proven to have good universality for different proteins. Second, the promoter of bacitracin synthetase gene cluster was replaced by these three promoters, and bacitraicn titer was enhanced by 14.62% when P Ubay was applied, which was decreased by 98.05% under the mediation of P Undh compared with that of the original strain DW2. Third, promoters P Ubay , P UyvgO , and P Undh were selected to regulate the expression levels of critical genes that are responsible for pucheriminic acid synthesis, and pucheriminic acid yield was increased by 194.1% via manipulating synthetic and competitive pathways. Finally, promoters P Ubay , P bacA , and P Undh were applied for green fluorescent protein (GFP) and RFP expression in Escherichia coli, and consistent effects were attained based on our results. Taken together, a gradient strength promoter library was constructed in this research, which provided an effective toolkit for fine-tuning gene expression and reprogramming metabolite metabolic flux in B. licheniformis. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Construction_and_Characterization_of_a_Gradient_Strength_Promoter_Library_for_Fine-Tuned_Gene_Expression_in_i_Bacillus_licheniformis_i_/16527107 |
| DOI: |
10.1021/acssynbio.1c00242.s001 |
| الاتاحة: |
https://doi.org/10.1021/acssynbio.1c00242.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.E7BCB282 |
| قاعدة البيانات: |
BASE |