Academic Journal
Functional analysis of the Landsberg erecta allele of FRIGIDA
| العنوان: | Functional analysis of the Landsberg erecta allele of FRIGIDA |
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| المؤلفون: | Schmalenbach, Inga, Zhang, Lei, Ryngajllo, Malgorzata, Jimenez-Gomez, José |
| المساهمون: | Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research (MPIPZ), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, German Research Foundation (DFG) (SCHM2793/1-1), Max Planck Society, International Max Planck Research School, German Research Foundation under German-Israeli Project Cooperation program (DFG DIP project) ( FE552/12-1) |
| المصدر: | ISSN: 1471-2229 ; BMC Plant Biology ; https://hal.science/hal-01204064 ; BMC Plant Biology, 2014, 14 (1), ⟨10.1186/s12870-014-0218-2⟩ ; http://www.biomedcentral.com/bmcplantbiol. |
| بيانات النشر: | HAL CCSD BioMed Central |
| سنة النشر: | 2014 |
| المجموعة: | Institut National de la Recherche Agronomique: ProdINRA |
| مصطلحات موضوعية: | Allelic series, Natural variation, Vernalization, FRIGIDA, Flowering time, Arabidopsis thaliana, [SDV]Life Sciences [q-bio] |
| الوصف: | Background: Most of the natural variation in flowering time in Arabidopsis thaliana can be attributed to allelic variation at the gene FRIGIDA (FRI, AT4G00650), which activates expression of the floral repressor FLOWERING LOCUS C (FLC, AT5G10140). Usually, late-flowering accessions carry functional FRI alleles (FRI-wt), whereas early flowering accessions contain non-functional alleles. The two most frequent alleles found in early flowering accessions are the ones present in the commonly used lab strains Columbia (FRI-Col) and Landsberg erecta (FRI-Ler), which contain a premature stop codon and a deletion of the start codon respectively. Results: Analysis of flowering time data from various Arabidopsis natural accessions indicated that the FRI-Ler allele retains some functionality. We generated transgenic lines carrying the FRI-Col or FRI-Ler allele in order to compare their effect on flowering time, vernalization response and FLC expression in the same genetic background. We characterize their modes of regulation through allele-specific expression and their relevance in nature through re-analysis of published datasets. We demonstrate that the FRI-Ler allele induces FLC expression, delays flowering time and confers sensitivity to vernalization in contrast to the true null FRI-Col allele. Nevertheless, the FRI-Ler allele revealed a weaker effect when compared to the fully functional FRI-wt allele, mainly due to reduced expression. Conclusions: The present study defines for the first time the existence of a new class of Arabidopsis accessions with an intermediate phenotype between slow and rapid cycling types. Although using available data from a common garden experiment we cannot observe fitness differences between accessions carrying the FRI-Ler or the FRI-Col allele, the phenotypic changes observed in the lab suggest that variation in these alleles could play a role in adaptation to specific natural environments. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-341317-5 0-00-341317-9 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/25207670; hal-01204064; https://hal.science/hal-01204064; https://hal.science/hal-01204064/document; https://hal.science/hal-01204064/file/2014_Schmalenbach_BMC%20Plant%20Biology_1.pdf; PRODINRA: 271009; PUBMED: 25207670; WOS: 000341317900001 |
| DOI: | 10.1186/s12870-014-0218-2 |
| الاتاحة: | https://hal.science/hal-01204064 https://hal.science/hal-01204064/document https://hal.science/hal-01204064/file/2014_Schmalenbach_BMC%20Plant%20Biology_1.pdf https://doi.org/10.1186/s12870-014-0218-2 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E7A1C18F |
| قاعدة البيانات: | BASE |
| ردمك: | 9780003413175 0003413179 |
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| DOI: | 10.1186/s12870-014-0218-2 |