Academic Journal
Mitochondrial DNA Damage in Earthworms: A Hazard Associated with Sublethal Systemic Pesticide Exposures
| العنوان: | Mitochondrial DNA Damage in Earthworms: A Hazard Associated with Sublethal Systemic Pesticide Exposures |
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| المؤلفون: | Chao Zeng, Qing Cheng, Wei Li, Shu Yan, Kaiye Li, Chensheng Lu |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biochemistry, Microbiology, Cell Biology, Neuroscience, Ecology, Immunology, Cancer, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, seed treatment applications, mitochondrial dna damage, highly significant increase, earthworm breeding facility, eisenia fetida <, posed mtdna toxicity, 001 ), either, mtdna toxicity, p <, vivo .<, triazole fungicide, systemic pesticides, sublethal exposures, soil biota, single neonic, levels left, increasing popularity, hazard associated, exposure period |
| الوصف: | The increasing popularity of seed treatment applications in agriculture may leave unintended hazards to soil biota, such as earthworms. The objective of this study was to explore mitochondrial DNA toxicity resulting from sublethal exposures to systemic pesticides, including four neonicotinoid insecticides (neonics), as well as coexposure to difenoconazole (DIF), a triazole fungicide, in earthworms ( Eisenia fetida ) in vivo. Earthworms were exposed under dose regimes resembling label recommendation and levels left in soil post seed treatment application for 30 days in an earthworm breeding facility. Mitochondrial DNA copy numbers (mtDNAcn) in earthworms were determined by using the 2 –ΔCt algorithm. Earthworms’ body weights were recorded before and after the exposure period. We found a highly significant increase of mtDNAcn in earthworms across all exposure groups (ANOVA, p < 0.001), either under a single neonic or combined with DIF exposure. Coupled with mtDNA toxicity, earthworms in the treatment groups gained significantly less weight than control earthworms (ANOVA, p < 0.001). We concluded that systemic pesticides, both neonics and DIF, posed mtDNA toxicity as measured by mtDNAcn, in earthworms. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Mitochondrial_DNA_Damage_in_Earthworms_A_Hazard_Associated_with_Sublethal_Systemic_Pesticide_Exposures/25218746 |
| DOI: | 10.1021/acs.estlett.3c00914.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.estlett.3c00914.s001 https://figshare.com/articles/journal_contribution/Mitochondrial_DNA_Damage_in_Earthworms_A_Hazard_Associated_with_Sublethal_Systemic_Pesticide_Exposures/25218746 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.E7A158A0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.estlett.3c00914.s001 |
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