Academic Journal
Integrated metabolomics and transcriptomics analysis of monolayer and neurospheres from established glioblastoma cell lines
| العنوان: | Integrated metabolomics and transcriptomics analysis of monolayer and neurospheres from established glioblastoma cell lines |
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| المؤلفون: | Peixoto, J, Janaki-Raman, S, Schlicker, L, Schmitz, W, Walz, S, Winkelkotte, AM, Herold-Mende, C, Soares, P, Schulze, A, Lima, J |
| المساهمون: | Instituto de Investigação e Inovação em Saúde |
| بيانات النشر: | MDPI |
| سنة النشر: | 2021 |
| المجموعة: | Repositório Aberto da Universidade do Porto |
| الوصف: | Altered metabolic processes contribute to carcinogenesis by modulating proliferation, survival and differentiation. Tumours are composed of different cell populations, with cancer stem-like cells being one of the most prominent examples. This specific pool of cells is thought to be responsible for cancer growth and recurrence and plays a particularly relevant role in glioblastoma (GBM), the most lethal form of primary brain tumours. Here, we have analysed the transcriptome and metabolome of an established GBM cell line (U87) and a patient-derived GBM stem-like cell line (NCH644) exposed to neurosphere or monolayer culture conditions. By integrating transcrip-tome and metabolome data, we identified key metabolic pathways and gene signatures that are associated with stem-like and differentiated states in GBM cells, and demonstrated that neuro-spheres and monolayer cells differ substantially in their metabolism and gene regulation. Further-more, arginine biosynthesis was identified as the most significantly regulated pathway in neuro-spheres, although individual nodes of this pathway were distinctly regulated in the two cellular systems. Neurosphere conditions, as opposed to monolayer conditions, cause a transcriptomic and metabolic rewiring that may be crucial for the regulation of stem-like features, where arginine bio-synthesis may be a key metabolic pathway. Additionally, TCGA data from GBM patients showed significant regulation of specific components of the arginine biosynthesis pathway, providing further evidence for the importance of this metabolic pathway in GBM. ; This work was funded by the European Regional Development Fund (ERDF) through the Operational Programme for Competitiveness and Internationalisation—COMPETE 2020, and Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia, under the project POCI-01-0145-FEDER-016390: CANCEL STEM. J.P. is financed through a grant from Fundação para a Ciência e a Tecnologia (SFRH/BD/105694/2015). I3S is an Associated Laboratory of the Portuguese ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6694 |
| Relation: | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F105694%2F2015/PT; Cancers, vol.13(6):1327; https://www.mdpi.com/2072-6694/13/6/1327; https://hdl.handle.net/10216/150484 |
| DOI: | 10.3390/cancers13061327 |
| الاتاحة: | https://hdl.handle.net/10216/150484 https://doi.org/10.3390/cancers13061327 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.E7803932 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20726694 |
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| DOI: | 10.3390/cancers13061327 |