Academic Journal

Antibody dynamics to SARS‐CoV‐2 in asymptomatic COVID‐19 infections

التفاصيل البيبلوغرافية
العنوان: Antibody dynamics to SARS‐CoV‐2 in asymptomatic COVID‐19 infections
المؤلفون: Lei, Qing, Li, Yang, Hou, Hong‐yan, Wang, Feng, Ouyang, Zhu‐qing, Zhang, Yandi, Lai, Dan‐yun, Banga Ndzouboukou, Jo‐Lewis, Xu, Zhao‐wei, Zhang, Bo, Chen, Hong, Xue, Jun‐biao, Lin, Xiao‐song, Zheng, Yun‐xiao, Yao, Zong‐jie, Wang, Xue‐ning, Yu, Cai‐zheng, Jiang, He‐wei, Zhang, Hai‐nan, Qi, Huan, Guo, Shu‐juan, Huang, Sheng‐hai, Sun, Zi‐yong, Tao, Sheng‐ce, Fan, Xiong‐lin
المساهمون: Research and Development, Shanghai Jiao Tong University, National Natural Science Foundation of China
المصدر: Allergy ; volume 76, issue 2, page 551-561 ; ISSN 0105-4538 1398-9995
بيانات النشر: Wiley
سنة النشر: 2020
المجموعة: Wiley Online Library (Open Access Articles via Crossref)
الوصف: Background The missing asymptomatic COVID‐19 infections have been overlooked because of the imperfect sensitivity of the nucleic acid testing (NAT). Globally understanding the humoral immunity in asymptomatic carriers will provide scientific knowledge for developing serological tests, improving early identification, and implementing more rational control strategies against the pandemic. Measure Utilizing both NAT and commercial kits for serum IgM and IgG antibodies, we extensively screened 11 766 epidemiologically suspected individuals on enrollment and 63 asymptomatic individuals were detected and recruited. Sixty‐three healthy individuals and 51 mild patients without any preexisting conditions were set as controls. Serum IgM and IgG profiles were further probed using a SARS‐CoV‐2 proteome microarray, and neutralizing antibody was detected by a pseudotyped virus neutralization assay system. The dynamics of antibodies were analyzed with exposure time or symptoms onset. Results A combination test of NAT and serological testing for IgM antibody discovered 55.5% of the total of 63 asymptomatic infections, which significantly raises the detection sensitivity when compared with the NAT alone (19%). Serum proteome microarray analysis demonstrated that asymptomatics mainly produced IgM and IgG antibodies against S1 and N proteins out of 20 proteins of SARS‐CoV‐2. Different from strong and persistent N‐specific antibodies, S1‐specific IgM responses, which evolved in asymptomatic individuals as early as the seventh day after exposure, peaked on days from 17 days to 25 days, and then disappeared in two months, might be used as an early diagnostic biomarker. 11.8% (6/51) mild patients and 38.1% (24/63) asymptomatic individuals did not produce neutralizing antibody. In particular, neutralizing antibody in asymptomatics gradually vanished in two months. Conclusion Our findings might have important implications for the definition of asymptomatic COVID‐19 infections, diagnosis, serological survey, public health, and ...
نوع الوثيقة: article in journal/newspaper
اللغة: English
DOI: 10.1111/all.14622
الاتاحة: http://dx.doi.org/10.1111/all.14622
https://onlinelibrary.wiley.com/doi/pdf/10.1111/all.14622
https://onlinelibrary.wiley.com/doi/full-xml/10.1111/all.14622
https://onlinelibrary.wiley.com/doi/am-pdf/10.1111/all.14622
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رقم الانضمام: edsbas.E7335EB9
قاعدة البيانات: BASE