Academic Journal
Radiation-damage-induced phasing: a case study using UV irradiation with light-emitting diodes
| العنوان: | Radiation-damage-induced phasing: a case study using UV irradiation with light-emitting diodes |
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| المؤلفون: | de Sanctis, Daniele, Zubieta, Chloe, Felisaz, Franck, Caserotto, Hugo, Nanao, Max H. |
| المساهمون: | European Synchrotron Radiation Facility (ESRF), Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), European Molecular Biology Laboratory Grenoble (EMBL), Unit of Virus Host Cell Interactions = Biologie structurale des interactions entre virus et cellule hôte (UVHCI), Université Joseph Fourier - Grenoble 1 (UJF)-European Molecular Biology Laboratory Grenoble (EMBL)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 2059-7983 ; EISSN: 2059-7983. |
| بيانات النشر: | CCSD International Union of Crystallography |
| سنة النشر: | 2016 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | radiation damage, ultraviolet light, UV, RIP, SIR, disulfide bonds, light-emitting diodes, phasing, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] |
| الوصف: | International audience ; Exposure to X-rays, high-intensity visible light or ultraviolet radiation results in alterations to protein structure such as the breakage of disulfide bonds, the loss of electron density at electron-rich centres and the movement of side chains. These specific changes can be exploited in order to obtain phase information. Here, a case study using insulin to illustrate each step of the radiation-damage-induced phasing (RIP) method is presented. Unlike a traditional X-ray-induced damage step, specific damage is introduced via ultraviolet light-emitting diodes (UV-LEDs). In contrast to UV lasers, UV-LEDs have the advantages of small size, low cost and relative ease of use |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-372366-3 0-00-372366-6 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/26960126; PRODINRA: 353389; PUBMED: 26960126; PUBMEDCENTRAL: PMC4784670; WOS: 000372366600012 |
| DOI: | 10.1107/S2059798315021658 |
| الاتاحة: | https://hal.science/hal-01572920 https://hal.science/hal-01572920v1/document https://hal.science/hal-01572920v1/file/Acta_Cryst_D_72_395.pdf https://doi.org/10.1107/S2059798315021658 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E7163BC2 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780003723663 0003723666 |
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| DOI: | 10.1107/S2059798315021658 |