Academic Journal
Mutational analysis of BTAF1–TBP interaction: BTAF1 can rescue DNA-binding defective
| العنوان: | Mutational analysis of BTAF1–TBP interaction: BTAF1 can rescue DNA-binding defective |
|---|---|
| المؤلفون: | Marcin P. Klejman, Xuemei Zhao, Frederik M. A. Van Schaik, Winship Herr |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://nar.oxfordjournals.org/content/33/17/5426.full.pdf. |
| سنة النشر: | 2005 |
| المجموعة: | CiteSeerX |
| الوصف: | The BTAF1 transcription factor interacts with TATA-binding protein (TBP) to form the B–TFIID complex, which is involved in RNA polymerase II transcription. Here, we present an extensive mapping study of TBP residues involved in BTAF1 interaction. This shows that residues in the concave, DNA-binding surface of TBP are important for BTAF1 binding. In addition, BTAF1 interacts with residues in helix 2 on the convex side of TBP as assayed in protein–protein and in DNA-binding assays. BTAF1 drastically changes the TATA-box binding specificity of TBP, as it is able to recruit DNA-binding defective TBP mutants to both TATA-containing and TATA-less DNA. Interestingly, other helix 2 interacting factors, such as TFIIA and NC2, can also stabilize mutant TBP binding to DNA. In contrast, TFIIB which interacts with a distinct surface of TBP does not display this activity. Since many proteins contact helix 2 of TBP, this provides a molecular basis for mutually exclusive TBP interac-tions and stresses the importance of this structural element for eukaryotic transcription. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.579.5777; http://nar.oxfordjournals.org/content/33/17/5426.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.579.5777 http://nar.oxfordjournals.org/content/33/17/5426.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.E6B86C17 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |