Academic Journal
A bvrR/bvrS Non-Polar Brucella abortus Mutant Confirms the Role of the Two-Component System BvrR/BvrS in Virulence and Membrane Integrity
| العنوان: | A bvrR/bvrS Non-Polar Brucella abortus Mutant Confirms the Role of the Two-Component System BvrR/BvrS in Virulence and Membrane Integrity |
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| المؤلفون: | Rivas-Solano, Olga, Núñez-Montero, Kattia, Altamirano-Silva, Pamela, Ruiz-Villalobos, Nazareth, BARQUERO-CALVO, ELIAS, Moreno, Edgardo, Chaves-Olarte, Esteban, Guzman-Verri, Caterina |
| المصدر: | Microorganisms, 11(8): 2014; Ago. 2023 |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Universidad Nacional Costa Rica (UNA): Repositorio Institucional |
| مصطلحات موضوعية: | BRUCELLOSIS, BRUCELOSIS, ZOONOSES, ZOONOSIS, BACTERIAS, ORGANISMOS PATOGENOS, BOVINOS |
| الوصف: | Brucella abortus is a bacterial pathogen causing bovine brucellosis worldwide. This facultative extracellular–intracellular pathogen can be transmitted to humans, leading to a zoonotic disease. The disease remains a public health concern, particularly in regions where livestock farming is present. The two-component regulatory system BvrR/BvrS was described by isolating the attenuated transposition mutants bvrR::Tn5 and bvrS::Tn5, whose characterization led to the understanding of the role of the system in bacterial survival. However, a phenotypic comparison with deletion mutants has not been performed because their construction has been unsuccessful in brucellae and difficult in phylogenetically related Rhizobiales with BvrR/BvrS orthologs. Here, we used an unmarked gene excision strategy to generate a B. abortus mutant strain lacking both genes, called B. abortus ∆bvrRS. The deletion was verified through PCR, Southern blot, Western blot, Sanger sequencing, and whole-genome sequencing, confirming a clean mutation without further alterations at the genome level. B. abortus ∆bvrRS shared attenuated phenotypic traits with both transposition mutants, confirming the role of BvrR/BvrS in pathogenesis and membrane integrity. This B. abortus ∆bvrRS with a non-antimicrobial marker is an excellent tool for continuing studies on the role of BvrR/BvrS in the B. abortus lifestyle. ; Brucella abortus es un patógeno bacteriano causante de la brucelosis bovina en todo el mundo. Este patógeno extracelular-intracelular facultativo puede transmitirse al ser humano, dando lugar a una enfermedad zoonótica. La enfermedad sigue siendo motivo de preocupación para la salud pública, sobre todo en las regiones donde hay ganadería. El sistema regulador de dos componentes BvrR/BvrS se describió aislando los mutantes de transposición atenuados mutantes de transposición atenuados bvrR::Tn5 y bvrS::Tn5, cuya caracterización condujo a la comprensión de el papel del sistema en la supervivencia bacteriana. Sin embargo, la comparación fenotípica ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://hdl.handle.net/11056/26175; https://doi.org/10.3390/microorganisms11082014 |
| DOI: | 10.3390/microorganisms11082014 |
| الاتاحة: | http://hdl.handle.net/11056/26175 https://doi.org/10.3390/microorganisms11082014 |
| Rights: | Acceso abierto ; Atribución-NoComercial-CompartirIgual 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-sa/4.0/ |
| رقم الانضمام: | edsbas.E6A7270C |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/microorganisms11082014 |
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