Academic Journal
Structure of HIV-1 RRE stem-loop II identifies two conformational states of the high-affinity Rev binding site
| العنوان: | Structure of HIV-1 RRE stem-loop II identifies two conformational states of the high-affinity Rev binding site |
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| المؤلفون: | Tipo, Jerricho, Gottipati, Keerthi, Slaton, Michael, Gonzalez-Gutierrez, Giovanni, Choi, Kyung H. |
| المساهمون: | University of Texas Medical Branch |
| المصدر: | Nature Communications ; volume 15, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2024 |
| الوصف: | During HIV infection, specific RNA-protein interaction between the Rev response element (RRE) and viral Rev protein is required for nuclear export of intron-containing viral mRNA transcripts. Rev initially binds the high-affinity site in stem-loop II, which promotes oligomerization of additional Rev proteins on RRE. Here, we present the crystal structure of RRE stem-loop II in distinct closed and open conformations. The high-affinity Rev-binding site is located within the three-way junction rather than the predicted stem IIB. The closed and open conformers differ in their non-canonical interactions within the three-way junction, and only the open conformation has the widened major groove conducive to initial Rev interaction. Rev binding assays show that RRE stem-loop II has high- and low-affinity binding sites, each of which binds a Rev dimer. We propose a binding model, wherein Rev-binding sites on RRE are sequentially created through structural rearrangements induced by Rev-RRE interactions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-024-48162-y |
| الاتاحة: | http://dx.doi.org/10.1038/s41467-024-48162-y https://www.nature.com/articles/s41467-024-48162-y.pdf https://www.nature.com/articles/s41467-024-48162-y |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.E67EF250 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-024-48162-y |
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