Academic Journal
NAADP mediates ATP-induced Ca2+ signals in astrocytes.
| العنوان: | NAADP mediates ATP-induced Ca2+ signals in astrocytes. |
|---|---|
| المؤلفون: | Barceló-Torns, M, Lewis, A, Gubern, A, Barneda, D, Bloor-Young, D, Picatoste, F, Churchill, G, Claro, E, Masgrau, R |
| سنة النشر: | 2016 |
| المجموعة: | Oxford University Research Archive (ORA) |
| الوصف: | Intracellular Ca(2+) signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca(2+) in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca(2+) responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://ora.ox.ac.uk/objects/uuid:af0684c2-fe2c-4862-b320-e2cd3898ea0a; https://doi.org/10.1016/j.febslet.2011.05.062 |
| DOI: | 10.1016/j.febslet.2011.05.062 |
| الاتاحة: | https://doi.org/10.1016/j.febslet.2011.05.062 https://ora.ox.ac.uk/objects/uuid:af0684c2-fe2c-4862-b320-e2cd3898ea0a |
| Rights: | info:eu-repo/semantics/embargoedAccess |
| رقم الانضمام: | edsbas.E65A4757 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.febslet.2011.05.062 |
|---|