Academic Journal
The Effect of Link N on the Differentiation of Human Mesenchymal Stem Cells
| العنوان: | The Effect of Link N on the Differentiation of Human Mesenchymal Stem Cells |
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| المؤلفون: | Mwale, F., Wang, H. T., Haglund, L., Roughley, P. J., Antoniou, J. |
| المصدر: | Global Spine Journal ; volume 2, issue 1_suppl, page s-0032-1320001-s-0032-1320001 ; ISSN 2192-5682 2192-5690 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2012 |
| الوصف: | Introduction The degeneration of intervertebral disk (IVD) can cause low back pain, and invoking the regeneration of degenerated IVD can relieve the pain. 1,2 Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into chondrocytes and can be employed in cartilage regeneration. 3,4 Link N (DHLSDNYTLDHDRAIH) is the amino terminal peptide of link protein in the extracellular matrix of cartilage. It can stimulate the biosynthesis of collagens II and IX and aggrecan in IVD cells in vitro as well as in vivo. 5–7 We hypothesized that Link N can enhance the chondrogenic differentiation of MSCs, and can be applied with MSCs in bio-regeneration of IVDs. The specific aim of this research is to determine the effect of Link N on gene expression of MSCs. Materials and Methods MSCs were isolated from the bone marrow of osteoarthritis (OA) patients. The cells were cultured in 24-well plates (3000 cells/well) in chondrogenesis differentiation medium (Invitrogen, Canada) according to the manufacturer's instructions. Link N was dissolved in the media with a final concentration of 0.1 µg/mL and 1 µg/mL, respectively. Medium without Link N was applied as a control. The media were changed every 3 days, and the used media were collected and stored at −20°C for GAG analysis. For gene expression analysis, the cells were cultured for 7, 14, and 21 days, then were washed twice with PBS and dissolved in Trizol (Invitrogen, Canada). Total RNA was isolated and cDNA was synthesized by reverse transcription. Gene expression was analyzed by real-time PCR and relative expression was calculated using GAPDH as a reference gene. MSCs from three OA patients were analyzed and statistical significance was calculated using analysis of variance followed by Fisher protected least significant difference (PLSD) comparison test. Results With the concentrations of 0.1 and 1.0 µg/mL, no toxic effect of Link N on MSCs was observed. After MSCs were cultured for 7 days and 14 days, the expression of aggrecan (ACAN) (Fig. 1), collagen II ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1055/s-0032-1320001 |
| الاتاحة: | http://dx.doi.org/10.1055/s-0032-1320001 http://journals.sagepub.com/doi/pdf/10.1055/s-0032-1320001 |
| Rights: | http://journals.sagepub.com/page/policies/text-and-data-mining-license |
| رقم الانضمام: | edsbas.E644F5C3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1055/s-0032-1320001 |
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