Academic Journal
Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models
| العنوان: | Venomics of the Duvernoy's gland secretion of the false coral snake Rhinobothryum bovallii (Andersson, 1916) and assessment of venom lethality towards synapsid and diapsid animal models |
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| المؤلفون: | Calvete Chornet, Juan José, Bonilla Murillo, Fabián, Granados Martínez, Sofía, Sanz, Libia, Lomonte, Bruno, Sasa Marín, Mahmood |
| المصدر: | Journal of Proteomics, vol.225, pp.1-11 |
| سنة النشر: | 2020 |
| المجموعة: | Universidad de Costa Rica: Repositorio Kérwá |
| مصطلحات موضوعية: | Snake venomics, Rear-fanged snake, Rhinobothryum bovallii, Duvernoy's gland secretion, Toxicovenomics, Diapsid-specific venom toxin, Colubridae 3FTx |
| الوصف: | The Duvernoy's gland secretory proteome of the false coral snake Rhinobothryum bovallii (Costa Rica), unveiled applying bottom-up venomics, comprises a handful of toxins belonging to only three protein families, three-finger toxin (3FTx), cysteine-rich secretory protein (CRISP), and snake venom metalloprotease (PIII-SVMP). Except for small differences in the relative abundance of the PIII-SVMPs, which may be due to individual variability, no evidence of geographic variability or ontogenetic changes was found among the venom proteomes of the juvenile and adult R. bovallii snakes sampled. Major monomeric (86.5%mol) and minor dimeric (2.8%mol) 3FTxs dominate the toxin arsenal of the Costa Rican false coral snake. The remaining 10.7% of the venom proteome comprises CRISP (8.2%) and PIII-SVMP (2.4%) molecules. In vivo lethality assays showed that R. bovallii produces venom that is non-toxic towards mammalian prey, and which exerts a different toxic effect on domestic chicken chicks and baby green iguana. Toxicovenomic analysis of R. bovallii venom in the iguana model identified two 3FTx RP-HPLC fractions that faithfully mimicked the irreversible immobilizing effect of the whole venom. ; Ministerio de Ciencia, Innovación y Universidades/[BFU2017-89103-P]/MICIU/España ; UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) ; UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biología |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-1-874391-92-0 1-874391-92-0 |
| تدمد: | 1874-3919 |
| Relation: | https://www.sciencedirect.com/science/article/abs/pii/S1874391920302505?via%3Dihub; https://hdl.handle.net/10669/83139 |
| DOI: | 10.1016/j.jprot.2020.103882 |
| الاتاحة: | https://hdl.handle.net/10669/83139 https://www.sciencedirect.com/science/article/abs/pii/S1874391920302505?via%3Dihub https://doi.org/10.1016/j.jprot.2020.103882 |
| رقم الانضمام: | edsbas.E5428EC7 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781874391920 1874391920 |
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| تدمد: | 18743919 |
| DOI: | 10.1016/j.jprot.2020.103882 |