Report
Persistently Elevated HBV Viral-Host Junction DNA in Urine as a Biomarker for Hepatocellular Carcinoma Minimum Residual Disease and Recurrence: A Pilot Study
| العنوان: | Persistently Elevated HBV Viral-Host Junction DNA in Urine as a Biomarker for Hepatocellular Carcinoma Minimum Residual Disease and Recurrence: A Pilot Study |
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| المؤلفون: | Lin, Selena Y, Halegoua-De Marzio, Dina, Block, Peter, Kao, Yu-Lan, Civan, Jesse M., Shieh, Fwu-Shan, Song, Wei, Hann, Hie-Won, Su, Ying-Hsiu |
| المصدر: | Department of Medicine Faculty Papers |
| بيانات النشر: | Jefferson Digital Commons |
| سنة النشر: | 2023 |
| المجموعة: | Jefferson Digital Commons (Thomas Jefferson University, Philadelphia) |
| مصطلحات موضوعية: | HBV integration, minimal residual disease, urine cell-free DNA, hepatocellular carcinoma, recurrence monitoring, Gastroenterology, Hepatology, Translational Medical Research |
| الوصف: | Hepatitis B virus (HBV)-host junction sequences (HBV-JSs) has been detected in the urine of patients with HBV infection. This study evaluated HBV-JSs as a marker of minimum residual disease (MRD) and tumor recurrence after treatment in HBV-hepatocellular carcinoma (HCC) patients. Archived serial urine DNA from two HBV–HCC with recurrence as confirmed by MRI and four HBV-related cirrhosis (LC) patients were used. Urinary HBV-JSs were identified by an HBV-targeted NGS assay. Quantitative junction-specific PCR assays were developed to investigate dynamic changes of the most abundant urinary HBV-JS. Abundant urinary HBV-JSs were identified in two cases of tumor recurrence. In case 1, a 78-year-old female with HBV- HCC underwent a follow-up MRI following microwave ablation. While MRI results were variable, the unique HBV-JS DNA, HBV-Chr17, steadily increased from initial diagnosis to HCC recurrence. In case 2, a 74-year-old male with HBV–HCC contained two HBV-JS DNA, HBV-Chr11 and HBV-TERT, that steadily increased after initial HCC diagnosis till recurrence. One LC examined had HBV-TERT DNA detected, but transiently in 3.5 years during HCC surveillance. HBV-JS DNA was persistently elevated prior to the diagnosis of recurrent HCC, suggesting the potential of urinary HBV-JS DNA to detect MRD and HCC recurrence after treatment. |
| نوع الوثيقة: | report |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://jdc.jefferson.edu/medfp/411; https://jdc.jefferson.edu/context/medfp/article/1434/viewcontent/Elevated_HBV_viral_host_junction_DNA_in_Urine.pdf; https://jdc.jefferson.edu/context/medfp/article/1434/filename/0/type/additional/viewcontent/Figure_S1_and_Table_S1.pdf |
| الاتاحة: | https://jdc.jefferson.edu/medfp/411 https://jdc.jefferson.edu/context/medfp/article/1434/viewcontent/Elevated_HBV_viral_host_junction_DNA_in_Urine.pdf https://jdc.jefferson.edu/context/medfp/article/1434/filename/0/type/additional/viewcontent/Figure_S1_and_Table_S1.pdf |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.E52204B3 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |