Academic Journal
Evaluation on Intestinal Permeability of Phlorotannins Using Caco-2 Cell Monolayers
| العنوان: | Evaluation on Intestinal Permeability of Phlorotannins Using Caco-2 Cell Monolayers |
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| المؤلفون: | Murata, Naoki, Keitoku, Saki, Miyake, Hideo, Tanaka, Reiji, Shibata, Toshiyuki |
| المساهمون: | New Energy and Industrial Technology Development Organization (NEDO) in Japan |
| المصدر: | Natural Product Communications ; volume 17, issue 1 ; ISSN 1934-578X 1555-9475 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2022 |
| الوصف: | Among the phlorotannins of seaweed polyphenols, eckols which have a dibenzodioxin linkage are known to have various physiological functions. The purpose of this study was to investigate the intestinal epithelial absorption of eckols using Caco-2 cell monolayers of the small intestinal membrane model. Each compound permeated from the apical (AP) side to the basolateral (BL) side in the monolayers was identified and quantitated by liquid chromatography-mass spectrometry with electrospray ionization. In the transport assays using five types of eckols (eckol, fucofuroeckol A, phlorofucofuroeckol A, dieckol, and 8,8'-bieckol), only the monomeric eckol showed limited transepithelial absorption with relatively small apparent permeability values (0.30 ± 0.04 × 10 −8 cm/s). Analyzing the Hanks’ balanced salt solution in the receiver on the BL side showed that phloroglucinol was detected in all experimental sections using eckols, and it's concentration increased with time over the course of the incubation. The other molecules corresponding to the unconjugated and conjugated metabolites of eckols were not detected in the AP and BL sides through the assays. These results suggest that eckols, including monomeric eckol, may be decomposed into phloroglucinol in the intestinal epithelium and the resulting phloroglucinol permeates to the BL side. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/1934578x211070415 |
| DOI: | 10.1177/1934578X211070415 |
| الاتاحة: | https://doi.org/10.1177/1934578x211070415 https://journals.sagepub.com/doi/pdf/10.1177/1934578X211070415 https://journals.sagepub.com/doi/full-xml/10.1177/1934578X211070415 |
| Rights: | https://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.E51431A5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/1934578x211070415 |
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